犬肿瘤细胞系中的 DNA 非整倍体和中心体扩增。

DNA aneuploidy and centrosome amplification in canine tumor cell lines.

机构信息

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Laboratory of Functional Genomics, Department of Medical Genome Science, Graduate School of Frontier Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.

出版信息

Tissue Cell. 2019 Dec;61:67-71. doi: 10.1016/j.tice.2019.09.003. Epub 2019 Sep 19.

DOI:10.1016/j.tice.2019.09.003

PMID:31759409

Abstract

DNA aneuploidy, the altered DNA content of a cell, is a common feature of canine tumors. However, it is unclear whether aneuploid DNA in canine tumor cells show centrosome amplification (CA), which contributes to numerical and structural chromosome aberrations that result in DNA aneuploidy. Here, we evaluated whether DNA aneuploidy and CA occur concurrently in canine tumor cell lines. Centrosome numbers were evaluated in 18 canine tumor cell lines by immunocytochemistry with anti-γ-tubulin antibody, and DNA content was evaluated by flow cytometry using propidium iodide. A total of 15 cell lines showed DNA aneuploidy, and CA was observed in 5 of these 15 cell lines. Together, our results suggest that DNA aneuploidy in canine tumor cells might be explained at least in part by CA. In addition, cell lines with CA may be useful tools to examine the detailed relationship between CA and DNA aneuploidy and the molecular mechanism of CA in canine tumor.

摘要

DNA 非整倍性是指细胞内 DNA 含量的改变，是犬肿瘤的一个常见特征。然而，犬肿瘤细胞中的非整倍体 DNA 是否表现出中心体扩增（CA），这有助于导致 DNA 非整倍性的数值和结构染色体畸变，目前尚不清楚。在这里，我们评估了犬肿瘤细胞系中是否同时发生 DNA 非整倍性和 CA。通过用抗γ-微管蛋白抗体进行免疫细胞化学，评估了 18 株犬肿瘤细胞系中的中心体数量，并通过使用碘化丙啶的流式细胞术评估了 DNA 含量。总共 15 株细胞系显示出 DNA 非整倍性，其中 5 株细胞系观察到 CA。总之，我们的结果表明，犬肿瘤细胞中的 DNA 非整倍性至少部分可以用 CA 来解释。此外，具有 CA 的细胞系可能是研究 CA 与 DNA 非整倍性之间的详细关系以及犬肿瘤中 CA 的分子机制的有用工具。