Nishioka Y
Department of Biology, McGill University, Montreal, Quebec, Canada.
Teratology. 1988 Aug;38(2):181-5. doi: 10.1002/tera.1420380211.
Identification of sex is often necessary to evaluate genetic or teratogenetic effects on embryonic development. A simple molecular technique to identify the sex of mouse embryos was studied using a Y chromosomal repetitive sequence (designated 145SC5). Since this technique does not require purification of DNA, it is particularly suitable for processing many embryos. Furthermore, 145SC5 detects 1% contamination of male DNA in a male-female DNA mixture. These results suggest that 145SC5 is a powerful molecular tool in a variety of studies on mouse development.
确定性别对于评估基因或致畸因素对胚胎发育的影响通常是必要的。我们利用一个Y染色体重复序列（命名为145SC5）研究了一种鉴定小鼠胚胎性别的简单分子技术。由于该技术不需要纯化DNA，所以特别适合处理多个胚胎。此外，145SC5能检测出雌雄DNA混合物中1%的雄性DNA污染。这些结果表明，145SC5在小鼠发育的各种研究中是一种强大的分子工具。
