Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt.
Veterinary Serum and Vaccine Research Institute, Ministry of Agriculture, Egypt.
Acta Trop. 2020 Feb;202:105281. doi: 10.1016/j.actatropica.2019.105281. Epub 2019 Nov 21.
This study evaluated plant-based immune-adjuvants from crude extracts of Phoenix dactylifera and Mentha piperita as promising adjuvants for vaccines because of the limited side effects associated with plant extracts. In addition, Montanide™ ISA 201 previously used in vaccines in cattle. Eight different infectious coryza (IC) vaccines were prepared from three serovars [A (W strain and local strain), C (Modesto strain) and B (0222 strain)] with eight Avibacterium paragallinarum vaccines adjuvants formulae using liquid paraffin, Montanide™ ISA 71, Montanide™ ISA 201, and Montanide™ Gel adjuvants, P. dactylifera and M. piperita as immune-stimulants at a concentration of 1 mg and 2 mg incorporated with or without liquid paraffin oil as an adjuvant. These vaccines were applied in a chicken model. After a single immunization, the eight vaccine formulations were evaluated using the ELISA and Microplate agglutination test. Evidence of protection in the immunized birds was based on the results after challenge and bacterial isolation. The incorporation of the crude aqueous extract of P. dactylifera or M. piperita at a concentration of 2 mg in a liquid paraffin oil adjuvanted IC vaccine could be employed as an efficient adjuvant for chicken to IC vaccine to enhance immune responses. Also,Montanide™ ISA 201 may be the best adjuvant to be used to enhance the protective response against Av. paragallinarum. Our results confirm that aqueous extracts of M. piperita leaves and P. dactylifera fruit have immunomodulatory potentials in vivo and elevated serum antibodies against Av. Paragallinarum.
本研究评估了来自 Phoenix dactylifera 和 Mentha piperita 的植物性免疫佐剂粗提物，因为它们与植物提取物相关的副作用有限，因此作为疫苗佐剂很有前景。此外，Montanide™ ISA 201 先前已用于牛疫苗。用三种血清型（A（W 株和本地株）、C（Modesto 株）和 B（0222 株））的 8 种不同传染性鼻炎（IC）疫苗，用液体石蜡、Montanide™ ISA 71、Montanide™ ISA 201 和 Montanide™ Gel 佐剂，用 1 mg 和 2 mg 的浓度与或不与液体石蜡油一起作为佐剂的 P. dactylifera 和 M. piperita 作为免疫刺激剂，制备了 8 种禽败血支原体疫苗佐剂配方。这些疫苗在鸡模型中应用。在单次免疫后，使用 ELISA 和微量板凝集试验评估了这 8 种疫苗配方。免疫鸟类的保护证据基于攻毒和细菌分离后的结果。在液体石蜡油佐剂的 IC 疫苗中加入 2 mg 浓度的 P. dactylifera 或 M. piperita 粗水提物，可作为鸡传染性鼻炎疫苗的有效佐剂，增强免疫反应。此外，Montanide™ ISA 201 可能是增强对禽败血支原体保护反应的最佳佐剂。我们的结果证实，M. piperita 叶和 P. dactylifera 果实的水提物具有体内免疫调节潜力，并能提高针对禽败血支原体的血清抗体。
