Department of Methodology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Banacha 1B, 02-097 Warsaw, Poland.
Department of Methodology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Banacha 1B, 02-097 Warsaw, Poland.
J Plast Reconstr Aesthet Surg. 2020 Feb;73(2):222-230. doi: 10.1016/j.bjps.2019.10.011. Epub 2019 Oct 11.
The recently introduced polyethylene glycol (PEG) treatment restores axonal continuity after nerve injury, leading to rapid recovery of nerve function. The impact of PEG therapy on neuroregeneration has not yet been compared with any intervention with an established proneuroregenerative potential. FK-506 is an immunosuppressive agent with documented proneuroregenerative potential in nerve injury models. The aim of this study was to compare the effects of PEG therapy and preinjury FK-506 administration in rats with sciatic nerve transection injury. Four groups of male Sprague Dawley rats (seven per group) underwent sciatic nerve transection with primary repair. Group A received placebo injections, group B placebo injections and PEG treatment, group C FK-506 injections, and group D both FK-506 injections and PEG treatment. Clinical outcomes were assessed by the skin prick test and Sciatic Functional Index (SFI). Regenerated nerves underwent histomorphometric analysis. The histomorphometric analysis demonstrated that compared with the controls, nerve specimens from all treated groups showed signs of enhanced neuroregeneration (higher mean axonal area) (p < 0.001). The histomorphometric parameters for group D (PEG + FK-506), mean axonal area (p < 0.001) and axonal count (p > 0.05), were significantly better than those in the other study groups. The Form factor was closest to its optimal values in group B (p < 0.0001). At the end of the study, mean skin prick test scores in all treated groups were significantly higher than those in controls (p > 0.05). During the first postoperative week, PEG-treated rats (groups B and D) presented with higher values of the SFI than animals from groups A and C, but the difference was not statistically significant. Combined therapy with PEG and FK-506 seems to produce better neuroregeneration outcomes than a simple suture-based repair complemented with either PEG or FK-506 treatment.
最近引入的聚乙二醇（PEG）治疗在神经损伤后恢复轴突连续性，导致神经功能迅速恢复。PEG 治疗对神经再生的影响尚未与任何具有既定促神经再生潜力的干预措施进行比较。FK-506 是一种免疫抑制剂，在神经损伤模型中具有已证实的促神经再生潜力。本研究旨在比较 PEG 治疗和损伤前 FK-506 给药在坐骨神经横断损伤大鼠中的效果。四组雄性 Sprague Dawley 大鼠（每组七只）接受坐骨神经横断修复术。A 组接受安慰剂注射，B 组接受安慰剂注射和 PEG 治疗，C 组接受 FK-506 注射，D 组同时接受 FK-506 注射和 PEG 治疗。通过皮肤刺痛试验和坐骨神经功能指数（SFI）评估临床结果。再生神经进行组织形态计量学分析。组织形态计量学分析表明，与对照组相比，所有治疗组的神经标本均显示出增强的神经再生迹象（更高的平均轴突面积）（p<0.001）。D 组（PEG+FK-506）的组织形态计量学参数，即平均轴突面积（p<0.001）和轴突计数（p>0.05），明显优于其他研究组。形态因子在 B 组最接近其最佳值（p<0.0001）。研究结束时，所有治疗组的平均皮肤刺痛试验评分均明显高于对照组（p>0.05）。在术后第一周，PEG 治疗组（B 组和 D 组）的 SFI 值高于 A 组和 C 组的动物，但差异无统计学意义。PEG 和 FK-506 的联合治疗似乎比单纯的基于缝合的修复辅以 PEG 或 FK-506 治疗产生更好的神经再生效果。
