Wang C P, Burckart G J, Venkataramanan R
Clinical Pharmacokinetics Laboratory, University of Pittsburgh, School of Pharmacy, PA 15261.
Ther Drug Monit. 1988;10(3):306-9.
Cyclosporine has numerous metabolites that may contribute to its immunosuppressive or toxicological properties. An adequate chromatographic separation of these metabolites must be achieved in order to determine their concentrations in biological fluids or to collect sufficient pure compound to test for pharmacologic or toxicologic activity. We evaluated six high pressure liquid chromatography (HPLC) columns for their ability to separate bile-derived cyclosporine metabolites using the critical separation of metabolite 17 from metabolite 1, and metabolite 1 from metabolite 18 as criteria for performance. Two octadecylsilane columns, the LC-18 by Supelco and the Resolve C-18 by Waters, provided adequate metabolite separation in less than 50 min using an HPLC gradient system.
环孢素具有众多代谢产物，这些代谢产物可能对其免疫抑制或毒理学特性有影响。为了测定它们在生物体液中的浓度，或收集足够的纯化合物以测试其药理或毒理活性，必须实现这些代谢产物的充分色谱分离。我们以代谢物17与代谢物1的关键分离以及代谢物1与代谢物18的分离作为性能标准，评估了六种高压液相色谱（HPLC）柱分离胆汁来源的环孢素代谢产物的能力。两根十八烷基硅烷柱，即Supelco公司的LC - 18柱和Waters公司的Resolve C - 18柱，使用HPLC梯度系统在不到50分钟内实现了代谢产物的充分分离。
