新型小檗碱纳米胶体的制备及其通过 Sirt1/NF-κB 通路改善糖尿病大鼠创面愈合的作用。

Preparation of novel berberine nano-colloids for improving wound healing of diabetic rats by acting Sirt1/NF-κB pathway.

机构信息

Jinzhou Medical University, Jinzhou, 121001, People's Republic of China.

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, 610041, Chengdu, People's Republic of China.

出版信息

Colloids Surf B Biointerfaces. 2020 Mar;187:110647. doi: 10.1016/j.colsurfb.2019.110647. Epub 2019 Nov 14.

DOI:10.1016/j.colsurfb.2019.110647

PMID:31761520

Abstract

In the present work, novel berberine nano-colloids hydrogel (BNH) was prepared for improving wound healing of diabetic rats. Polyvinyl alcohol (PVA), sodium alginate (Alg) were adopted as building blocks to form BNH. Calcium ions was used as crosslink agent to construct BNH. The skin injury model of diabetic rats was successfully established, and the prepared hydrogel was applied to the wound. Animal experiments proved that BNH could promote wound healing of diabetic rats. Further molecular mechanism research revealed that BNH could inhibit the expression of NF-κB, TNF-a and IL-6, but increase the expression of F VEGF, CD 31 and SMA by activating Sirt 1 which were benefit for wound healing of diabetic rats.

摘要

在本工作中，为了改善糖尿病大鼠的伤口愈合，制备了新型小檗碱纳米胶体水凝胶（BNH）。采用聚乙烯醇（PVA）、海藻酸钠（Alg）作为构建块形成 BNH。钙离子作为交联剂构建 BNH。成功建立了糖尿病大鼠皮肤损伤模型，并将制备的水凝胶应用于伤口。动物实验证明 BNH 可以促进糖尿病大鼠的伤口愈合。进一步的分子机制研究表明，BNH 通过激活 Sirt 1 抑制 NF-κB、TNF-a 和 IL-6 的表达，同时增加 F VEGF、CD 31 和 SMA 的表达，这有利于糖尿病大鼠的伤口愈合。