紫阳平方促进糖尿病大鼠皮肤伤口愈合。
Zuyangping formula promotes skin wound healing in diabetic rats.
机构信息
Department of Pharmacy, Wuhan University Tongren Hospital (the Third Hospital of Wuhan), Wuhan 430060, China.
Department of Pharmacy, the First People's Hospital of Jiangxia District, Wuhan 430200, China.
出版信息
J Tradit Chin Med. 2024 Dec;44(6):1194-1203. doi: 10.19852/j.cnki.jtcm.2024.06.008.
OBJECTIVE
To evaluate the effects of Zuyangping (, ZYP) formula on wound healing in diabetic rats, as well as the molecular mechanisms involved.
METHODS
The main compounds in ZYP formula were identified by the Liquid chromatography-tandem mass spectrometry. Sprague-Dawley rats, injected with streptozotocin (STZ) to establish diabetes model, then, formed a defective skin trauma in the back, and each group was treated with corresponding drugs once a day. Granulation was taken from each time node for histological analysis. The Western blotting was used to measured protein expression of advanced glycation end products receptor (RAGE) and hypoxia-inducible factor-1α (HIF-1α) axis-related proteins. The relative expression levels of inflammatory cytokines and growth factors were measured by the enzyme-linked immunosorbent assay method.
RESULTS
The main ingredients were identified in the ZYP formula. Histological analysis showed that the ZYP formula could inhibit the expression of inflammation, promote angiogenesis and collagen deposition. In addition, the ZYP formula could regulate the expression of RAGE and HIF1-α axis-related proteins, thus promoting the wound healing in diabetic rats.
CONCLUSION
The ZYP formula could accelerate wound healing in diabetic rats.
目的
评价滋阴平阳（ZYP）方对糖尿病大鼠创面愈合的影响及其作用机制。
方法
采用液质联用技术鉴定 ZYP 方的主要成分。采用链脲佐菌素（STZ）诱导 Sprague-Dawley 大鼠建立糖尿病模型，背部制作皮肤缺损创面，每天给予相应药物处理。在不同时间点取肉芽组织进行组织学分析。采用 Western blot 法检测晚期糖基化终末产物受体（RAGE）和低氧诱导因子-1α（HIF-1α）轴相关蛋白的表达。采用酶联免疫吸附试验法检测炎症因子和生长因子的相对表达水平。
结果
鉴定了 ZYP 方中的主要成分。组织学分析表明，ZYP 方能够抑制炎症表达，促进血管生成和胶原沉积。此外，ZYP 方还可以调节 RAGE 和 HIF1-α 轴相关蛋白的表达，从而促进糖尿病大鼠的创面愈合。
结论
ZYP 方可以加速糖尿病大鼠的创面愈合。
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