Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Mar 5;228:117696. doi: 10.1016/j.saa.2019.117696. Epub 2019 Nov 4.
A new dual-responsive chiral cystine based chemosensor, Cys(cou), has been designed and characterized by H NMR, C NMR, FT-IR, UV-vis as well as elemental analysis. This sensor exhibited an excellent response towards Fe and CN with high selectivity and sensitivity by fluorescence turn-off mechanism. The binding mode of Cys(cou) with Fe, and CN was confirmed by ESI-MS, H NMR, and fluorescence titration and also quantum chemical calculation. These results showed that the stoichiometric ratio of Cys(cou)-Fe and Cys(cou)-CN is 1:1 and 1:3 in DMSO/Tris aqueous buffer (1:1, v/v), respectively. The linear relationship of the Stern-Volmer plot illustrates the static quenching mechanism at different concentrations. The detection limit (LOD) and binding constant (K) for Fe and CN are 0.029 μM, 1.28 × 10 and 0.51 μM, 9.94 × 10, respectively. Moreover, Cys(cou) can act as a colorimetric sensor for CN in DMSO with the color change from colorless to yellow.
一种新的双响应手性半胱氨酸基化学传感器 Cys(cou) 已经通过 1H NMR、13C NMR、FT-IR、UV-vis 和元素分析进行了设计和表征。该传感器通过荧光关闭机制表现出对 Fe 和 CN 的优异响应，具有高选择性和灵敏度。通过 ESI-MS、1H NMR 和荧光滴定以及量子化学计算，证实了 Cys(cou) 与 Fe 和 CN 的结合模式。结果表明，在 DMSO/Tris 水缓冲液（1:1，v/v）中，Cys(cou)-Fe 和 Cys(cou)-CN 的化学计量比分别为 1:1 和 1:3。Stern-Volmer 图的线性关系说明了不同浓度下的静态猝灭机制。Fe 和 CN 的检测限（LOD）和结合常数（K）分别为 0.029 μM、1.28×10 和 0.51 μM、9.94×10。此外，Cys(cou) 可以在 DMSO 中作为 CN 的比色传感器，颜色从无色变为黄色。
