[Study on the Expression of 6 of Osteoblast in Diabetic Environment].

OBJECTIVE

To investigate the ability of osteogenic differentiation and the expression of histone demethylases KDM6B in bone marrow mesenchymal stem cells (BMSCs) in diabetic environment.

METHODS

Diabetic model rats was successfully established, and BMSCs from diabetic model rats and normal rats were isolated and cultured for further study. When cultured cells, we added high concentration of glucose and advanced glycosylation products (AGE) in the medium to imitating the diabetic environment. BMSCs were divided into 6 groups: diabetes group (derived from diabets SD rats), normal group (derived from normal SD rats), high glucose group (30 mmol/L D-glucose), normal glucose group (5.5 mmol/L D-glucose), AGE group (AGE 300 g/mL) and BSA group (BSA 300 g/mL). BMSCs in diabetes group were derived from diabetes SD rats, while others were derived from normal SD rats. After 7 d of osteogenic induction, the cells were examined the ability of osteogenic differentiation by alkaline phosphatase (ALP) staining, the transcription levels of Runt-related transcription factor 2 () and 6 were determined by RT-PCR, and the expression levels of H3K27Me3 protein were examined by Western bolt.

RESULTS

Compared with the control groups, the numbers of ALP stained cells and the mRNA levels of and 6 in diabetes group, high glucose group and AGE group were all decreased ( < 0.05), while H3K27Me3 protein expression levels were all increased ( < 0.05).

CONCLUSION

The ability of osteogenic differentiation of BMSCs in diabetic environment was weakened, and the expression of mRNA was inhibited, which may be related to the increased expression of H3K27Me3 after the inhibition of 6 expression.