Tong Ka Yan, Zhao Jia, Tse Chun-Wai, Wan Pui-Ki, Rong Jianhui, Au-Yeung Ho Yu
The University of Hong Kong , State Key Laboratory for Synthetic Chemistry , Department of Chemistry , Pokfulam Road , P. R. China . Email:
School of Chinese Medicine , The University of Hong Kong , 10 Sassoon Road, Pokfulam , Hong Kong , P. R. China.
Chem Sci. 2019 Aug 14;10(37):8519-8526. doi: 10.1039/c9sc03338f. eCollection 2019 Oct 7.
The development of a new triggered-release system for selective detection of catecholamines in biological samples including living cells is reported. Catecholamines are a class of tightly regulated hormones and neurotransmitters in the human body and their dysregulation is implicated in various neurodegenerative diseases. It is highly challenging to selectively sense and detect catecholamines in a complex biological environment due to their small size, non-specific molecular shape and trivial chemical properties. In this study, a copper-based, catecholamine-triggered oxidation that releases a fluorescent reporter is described. The probe is highly sensitive and selective for detecting changes in catecholamine levels in aqueous buffer, human plasma, and cellular models of neuronal differentiation and Parkinson's disease. This new catecholamine sensing strategy features chemical reactivity as part of small molecule recognition as opposed to the conventional use of a well-designed host for reversible binding.
本文报道了一种用于选择性检测生物样品（包括活细胞）中儿茶酚胺的新型触发释放系统的开发。儿茶酚胺是人体中一类受到严格调控的激素和神经递质，其失调与多种神经退行性疾病有关。由于儿茶酚胺体积小、分子形状无特异性且化学性质平凡，因此在复杂的生物环境中选择性地感知和检测它们极具挑战性。在本研究中，描述了一种基于铜的、由儿茶酚胺触发氧化并释放荧光报告分子的系统。该探针对于检测水缓冲液、人血浆以及神经元分化和帕金森病细胞模型中儿茶酚胺水平的变化具有高度敏感性和选择性。这种新的儿茶酚胺传感策略的特点是将化学反应性作为小分子识别的一部分，这与传统上使用精心设计的主体进行可逆结合有所不同。
