在玻璃安瓿和玻璃毛细管中使用气相色谱-质谱联用仪对木质素H/G/S组成进行快速、简化的微量定量分析。

Rapid, simplified microscale quantitative analysis of lignin H/G/S composition with GC-MS in glass ampules and glass capillaries.

作者信息

Qi Q, Hu Jiaqi, Qu Long, Jiang Xiangning, Gai Yang, Valenzuela Sofia A, Qi Liwang

机构信息

College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.

College of Horticulture, China Agriculture University, Beijing, China.

出版信息

MethodsX. 2019 Nov 7;6:2592-2600. doi: 10.1016/j.mex.2019.11.005. eCollection 2019.

DOI:10.1016/j.mex.2019.11.005

PMID:31763191

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6861596/

Abstract

The characterization of monolignols H-, G- and S- units composition Vanholme et al. (2010) of lignin is important in agriculture, forestry, herb medicine, livestock, and health care research Vanholme et al. (2008) and Sticklen (2008). The conventional methods often require a great deal of samples and reagents and are time-consuming. Here, we present a newly developed method with fewer operations. The optimized method is suitable for detecting and characterizing lignin composition of cell wall in different plant species and has the advantages of: •Avoiding the influence of plasticizer by plasticware and enhancing the accuracy of monolignols analysis.•Lowering the required samples from grams to milligrams, and organic reagents from milliliters to microliters.•Reducing the time required from a few days to 6 h.

摘要

木质素中单木酚H-、G-和S-单元组成的表征（Vanholme等人，2010年）在农业、林业、草药、畜牧业和医疗保健研究中（Vanholme等人，2008年；Sticklen，2008年）具有重要意义。传统方法通常需要大量样品和试剂，且耗时较长。在此，我们提出一种操作较少的新开发方法。该优化方法适用于检测和表征不同植物物种细胞壁的木质素组成，具有以下优点：•避免塑料制品中增塑剂的影响，提高单木酚分析的准确性。•将所需样品从克降至毫克，有机试剂从毫升降至微升。•将所需时间从几天缩短至6小时。