Department of Environmental Engineering, Kyungpook National University, Daegu, 41566, Republic of Korea.
Biological Sciences Department, College of Science, King Faisal University, Saudi Arabia; Botany and Microbiology Department, Faculty of Science, University of Beni-Suef, Beni-Suef, Egypt.
Chemosphere. 2020 Mar;243:125389. doi: 10.1016/j.chemosphere.2019.125389. Epub 2019 Nov 18.
The present study is aimed to isolate and identify polycyclic aromatic hydrocarbons (PAHs) degrading bacteria from brackish water and to assess the biodegradation efficiency against low and high molecular weight PAHs. Among 15 isolates, the isolate designated as RM effectively degraded 100 mg/L of phenanthrene (Phe) (67.0%), pyrene (Pyr) (63.0%), naphthalene (NaP) (60.0%), and benzo [a]pyrene (BaP) (58.0%) after 7 days of incubation. Carbon sources, pH, and salinity of the culture medium were optimized to enhance the growth and PAHs biodegradation of the isolate RM. Sucrose was found to be an excellent carbon source to enhance PAHs biodegradation (Phe, 75.0; Pyr, 68.5; NaP, 62.5; and BaP, 59.5%). Furthermore, the isolate showed enhanced degradation at pH 7.0 and 4% salinity. The isolate RM was identified as Halomonas sp. based on partial 16S rDNA gene sequence analysis. The results indicated that the isolate RM (i.e., Halomonas sp.) has the potential to be used in remediation of oil spills in the marine ecosystem.
本研究旨在从咸水中分离和鉴定多环芳烃（PAHs）降解菌，并评估其对低分子量和高分子量 PAHs 的生物降解效率。在 15 个分离株中，命名为 RM 的分离株在 7 天的培养后，有效地降解了 100mg/L 的菲（Phe）（67.0%）、芘（Pyr）（63.0%）、萘（NaP）（60.0%）和苯并[a]芘（BaP）（58.0%）。优化了培养物的碳源、pH 值和盐度，以增强分离株 RM 的生长和 PAHs 生物降解能力。发现蔗糖是增强 PAHs 生物降解的极好碳源（Phe 为 75.0%；Pyr 为 68.5%；NaP 为 62.5%；BaP 为 59.5%）。此外，该分离株在 pH 值为 7.0 和盐度为 4%时表现出增强的降解能力。根据部分 16S rDNA 基因序列分析，分离株 RM 被鉴定为盐单胞菌属（Halomonas sp.）。结果表明，分离株 RM（即盐单胞菌属）具有在海洋生态系统中用于修复溢油的潜力。
