Vizethum W, Goerz G
Chem Biol Interact. 1979 Dec;28(2-3):291-9. doi: 10.1016/0009-2797(79)90169-8.
The application of hexachlorobenzene (HCB), pentachlorophenol (PCP) and 2,4,5-trichlorophenol (TCP) to female rats led to an induction of both the microsomal and the nuclear cytochrome P-450 system in the liver. The increase of th mixed-function hydroxylase activities examined (7-ethoxycoumarin deethylase, 7-ethoxyresorufin deethylase, NADPH-dependent cytochrome c reductase, aminopyrine demethylase, benzpyrene hydroxylase) did not correlate strictly with the cytochrome P-450 content. Depending on the inducers and the substrates used, the content and the activity of the cytochrome P-450 were essentially smaller in the nuclei than in the microsomes. It was striking that in the nuclei those activities (benzpyrene hydroxylase, 7-ethoxyresorufin deethylase, 7-ethoxycoumarin deethylase) were preferably induced which can be attributed to the methyl-cholanthrene-induced form of the cytochrome P-450 (cytochrome P-448). These results suggest, also in the light of findings of other authors, the induction of different species of cytochrome P-450 in the nuclei and microsomes.
将六氯苯（HCB）、五氯苯酚（PCP）和2,4,5-三氯苯酚（TCP）应用于雌性大鼠，可诱导肝脏中的微粒体和细胞核细胞色素P-450系统。所检测的混合功能羟化酶活性（7-乙氧基香豆素脱乙基酶、7-乙氧基试卤灵脱乙基酶、NADPH依赖性细胞色素c还原酶、氨基比林脱甲基酶、苯并芘羟化酶）的增加与细胞色素P-450含量并不严格相关。根据所使用的诱导剂和底物，细胞核中细胞色素P-450的含量和活性基本上低于微粒体中的含量和活性。值得注意的是，在细胞核中，那些可归因于甲基胆蒽诱导型细胞色素P-450（细胞色素P-－448）的活性（苯并芘羟化酶、7-乙氧基试卤灵脱乙基酶、7-乙氧基香豆素脱乙基酶）被优先诱导。根据其他作者的研究结果，这些结果也表明在细胞核和微粒体中诱导出了不同种类的细胞色素P-450。
