Department of Bioengineering, Zhuhai Campus, Zunyi Medical University, Zhuhai 519041, China.
Molecules. 2019 Nov 25;24(23):4283. doi: 10.3390/molecules24234283.
Dual-function chemosensors that combine the capability of colorimetric and fluorimetric detection of Cu are still relatively rare. Herein, we report that a 3-hydroxyflavone derivative ()-2-(4-(dimethylamino)styryl)-3-hydroxy-4-chromen-4-one (), which is a red-emitting fluorophore, could serve as a reversible colorimetric and fluorescence "turn-off" chemosensor for the detection of Cu. Upon addition of Cu to in neutral aqueous solution, a dramatic color change from yellow to purple-red was clearly observed, and its fluorescence was markedly quenched, which was attributed to the complexation between the chemosensor and Cu. Conditions of the sensing process had been optimized, and the sensing studies were performed in a solution of ethanol/phosphate buffer saline (/ = 3:7, pH = 7.0). The sensing system exhibited high selectivity towards Cu. The limit of naked eye detection of Cu was determined at 8 × 10 mol/L, whereas the fluorescence titration experiment showed a detection limit at 5.7 × 10 mol/L. The complexation between and Cu was reversible, and the binding constant was found to be 3.2 × 10 M. Moreover, bioimaging experiments showed that could penetrate the cell membrane and respond to the intracellular changes of Cu within living cells, which indicated its potential for biological applications.
双功能化学传感器，结合比色和荧光检测 Cu 的能力仍然相对较少。在此，我们报告说，3-羟基黄酮衍生物（）-2-（4-（二甲基氨基）-苯乙烯基）-3-羟基-4-色烯-4-酮（），它是一个红色发射荧光团，可作为一种可逆比色和荧光“关闭”化学传感器，用于检测 Cu。向中性水溶液中的添加 Cu 后，明显观察到从黄色到紫色红色的明显颜色变化，并且其荧光被明显猝灭，这归因于化学传感器与 Cu 的络合。优化了传感过程的条件，并在乙醇/磷酸盐缓冲盐水溶液（/ = 3:7，pH = 7.0）中进行了传感研究。传感系统对 Cu 表现出高选择性。Cu 的肉眼检测极限确定为 8×10 mol/L，而荧光滴定实验显示检测极限为 5.7×10 mol/L。与 Cu 的络合是可逆的，并且发现结合常数为 3.2×10 M。此外，生物成像实验表明可以穿透细胞膜并响应活细胞内 Cu 的细胞内变化，这表明其在生物应用中的潜力。
