State Key Laboratory of Transducer Technology, Institute of Electronics , Chinese Academy of Sciences , Beijing 100190 , PR China.
University of Chinese Academy of Sciences , Beijing 100049 , PR China.
ACS Sens. 2019 Dec 27;4(12):3186-3194. doi: 10.1021/acssensors.9b01633. Epub 2019 Dec 11.
Owing to its critical role in the development of female reproductive tissues and as a clinical biomarker, there is an urgent need to develop a rapid and cost-effective method to sensitively detect 17β-estradiol (E2). In this work, a folding aptasensor platform with microfluidic channels for the label-free electrochemical detection of E2 is described. The platform, designed with a delicate folding structure, integrating filter holes, microfluidic channels, reaction chambers, and a three-electrode system, is extremely easy to use. To increase the detection sensitivity and immobilize the aptamer, we synthesized a novel nanoassembly consisting of amine-functionalized single-walled carbon nanotube/new methylene blue/gold nanoparticles (AuNPs) and modified the working electrode with this nanoassembly. The calibration curve obtained from the experimental results exhibited a linear range between 10 pg mL and 500 ng mL ( = 0.993), and a detection limit of 5 pg mL was achieved (S/N = 3). Furthermore, experiments to detect E2 in clinical serum were conducted, and the results were highly similar to those obtained using a large electrochemical luminescence apparatus. By integrating multiple functional components, adopting novel nanoassemblies, and using a folding structure, this paper-based platform not only has great potential as a simple and convenient integrated device for point-of-care testing of E2, but also as a portable, low-cost, and highly sensitive aptasensor platform capable of detecting many diagnostic biomarkers with the appropriate aptamers.
由于其在女性生殖组织发育和作为临床生物标志物方面的关键作用，因此迫切需要开发一种快速且经济有效的方法来灵敏地检测 17β-雌二醇（E2）。在这项工作中，描述了一种带有微流道的折叠适体传感器平台，用于无标记电化学检测 E2。该平台采用精致的折叠结构设计，集成了过滤孔、微流道、反应室和三电极系统，非常易于使用。为了提高检测灵敏度并固定适体，我们合成了一种由胺功能化单壁碳纳米管/新亚甲基蓝/金纳米粒子（AuNPs）组成的新型纳米组装体，并使用该纳米组装体修饰工作电极。从实验结果得到的校准曲线显示出在 10 pg mL 至 500 ng mL 之间的线性范围（r = 0.993），并且达到了 5 pg mL 的检测限（S/N = 3）。此外，还进行了用于检测临床血清中 E2 的实验，并且得到的结果与使用大型电化学发光仪获得的结果高度相似。通过集成多个功能组件、采用新型纳米组装体和使用折叠结构，这种基于纸的平台不仅具有作为用于 E2 即时检测的简单方便的集成设备的巨大潜力，而且还具有作为便携式、低成本和高灵敏度的适体传感器平台的潜力，能够检测许多具有适当适体的诊断生物标志物。
