Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo.
Noda Institute for Scientific Research.
J Gen Appl Microbiol. 2020 Aug 26;66(3):195-199. doi: 10.2323/jgam.2019.07.001. Epub 2019 Nov 27.
We recently developed an Aspergillus oryzae strain in which malonyl-coenzyme A (CoA) supply is strengthened by the deletion of snfA and SCAP as an efficient host to produce a plant polyketide, curcumin. Here, we examined the effectiveness of this strain in producing another polyketide, atrochrysone carboxylic acid (ACA), which is synthesized from eight molecules of malonyl-CoA using an iterative type I polyketide synthase, ACA synthase (ACAS), and atrochrysone carboxyl ACP thioesterase (ACTE) in Aspergillus terreus. When ACAS and ACTE were introduced, the A. oryzae ΔsnfAΔSCAP strain produced approximately four times more ACA-related polyketides than did the control strain expressing both genes. This result further demonstrated the availability of the A. oryzae ΔsnfAΔSCAP strain for heterologous polyketide production.
我们最近开发了一株米曲霉菌株，通过缺失 snfA 和 SCAP 来增强丙二酰辅酶 A（CoA）的供应，作为生产植物聚酮化合物姜黄素的有效宿主。在这里，我们研究了该菌株在生产另一种聚酮化合物阿托曲沙酸羧酸（ACA）方面的有效性，ACA 是由 8 个丙二酰 CoA 分子使用迭代型 I 聚酮合酶、ACA 合酶（ACAS）和阿托曲沙酸羧基 ACP 硫酯酶（ACTE）在土曲霉中合成的。当引入 ACAS 和 ACTE 时，与表达这两个基因的对照菌株相比，米曲霉ΔsnfAΔSCAP 菌株产生了大约四倍的 ACA 相关聚酮化合物。这一结果进一步证明了米曲霉ΔsnfAΔSCAP 菌株在异源聚酮化合物生产中的可用性。
