Reduced Expression of Associates With Disruption of Glucose Sensing and Insulin Signaling in Pancreatic β-Cells.

Previous work has shown that reduced expression of , a member of the phosphoinositide-specific phospholipases (PI-PLC) family, impaired insulin secretion with an unclear mechanism. In the current study, we aim to investigate the mechanism underlying this effect using human islets and rat INS-1 (832/13) cells. Microarray and RNA sequencing data showed that is among the highly expressed PI-PLCs in human islets and INS-1 (832/13) cells. Expression of was reduced in human diabetic islets, correlated positively with and expression and inversely with the donor's body mass index (BMI) and glycated hemoglobin (HbA). Expression silencing of in INS-1 (832/13) cells was found to reduce glucose-stimulated insulin secretion (GSIS) and insulin content. In addition, the expression of , and was downregulated. Cell viability and apoptosis rate were unaffected. In conclusion, our data suggest that low expression of in pancreatic β-cells associates with downregulation of the key insulin signaling and insulin biosynthesis genes as well as reduction in glucose sensing.