Liu Zhiyuan, Xiao Shune, Tao Kerong, Li Hai, Jin Wenhu, Wei Zairong, Wang Dali, Deng Chengliang
Department of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.
Stem Cells Int. 2019 Oct 30;2019:3091619. doi: 10.1155/2019/3091619. eCollection 2019.
Pressure injury (PI) affects quality of life and results in economic and social burdens. Local transplantation of human adipose-derived stem cells (ASCs) is considered an effective treatment. However, ASC suspension alone is vulnerable to the immune system and results in a shortened cell survival. There is increasing evidence of a synergistic effect of platelet-rich plasma (PRP) combined with ASCs on wound healing. This study investigated the effectiveness, synergy, and mechanism of wound healing following local injection of PRP combined with ASCs in a rodent PI model. PRP or ASCs alone were the control intervention. Wound healing, inflammatory infiltration, collagen deposition, angiogenesis, neurogenesis, and cell homing were investigated. PI healing was promoted by the synergistic effects of PRP combined with ASCs. The combination was more effective than ASCs alone for modulating inflammation, increasing collagen deposition, angiogenesis, neurogenesis, and the persistence of the injected ASCs. These data provide a theoretical foundation for the clinical administration of ASCs combined with PRP in PI healing and skin regeneration.
压力性损伤（PI）会影响生活质量，并导致经济和社会负担。人脂肪来源干细胞（ASC）的局部移植被认为是一种有效的治疗方法。然而，单纯的ASC悬液易受免疫系统影响，导致细胞存活时间缩短。越来越多的证据表明，富含血小板血浆（PRP）与ASC联合应用对伤口愈合具有协同作用。本研究在啮齿动物PI模型中，探讨局部注射PRP与ASC联合应用后伤口愈合的有效性、协同作用及机制。单独使用PRP或ASC作为对照干预。研究了伤口愈合、炎症浸润、胶原沉积、血管生成、神经发生和细胞归巢情况。PRP与ASC联合应用的协同作用促进了PI愈合。该联合应用在调节炎症、增加胶原沉积、血管生成、神经发生以及注射的ASC的持久性方面比单独使用ASC更有效。这些数据为ASC与PRP联合应用于PI愈合和皮肤再生的临床给药提供了理论基础。
