Department of Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Int J Radiat Oncol Biol Phys. 2010 Nov 1;78(3):888-96. doi: 10.1016/j.ijrobp.2010.05.002. Epub 2010 Aug 12.
A delayed full-thickness wound-healing model was developed and used for examining the capacity of adipose-derived stem cells (ASCs), either alone or in platelet-rich fibrin gels, to promote healing.
Four pigs received electron beam radiation to the dorsal skin surface. Five weeks after radiation, subcutaneous fat was harvested from nonirradiated areas and processed to yield ASCs. Two weeks later, 28 to 30 full-thickness 1.5-cm(2) wounds were made in irradiated and nonirradiated skin. Wounds were treated with either saline solution, ASCs in saline solution, platelet-rich plasma (PRP) fibrin gel, ASCs in PRP, or non-autologous green fluorescence protein-labeled ASCs.
The single radiation dose produced a significant loss of dermal microvasculature density (75%) by 7 weeks. There was a significant difference in the rate of healing between irradiated and nonirradiated skin treated with saline solution. The ASCs in PRP-treated wounds exhibited a significant 11.2% improvement in wound healing compared with saline solution. Enhancement was dependent on the combination of ASCs and PRP, because neither ASCs nor PRP alone had an effect.
We have created a model that simulates the clinically relevant late radiation effects of delayed wound healing. Using this model, we showed that a combination of ASCs and PRP improves the healing rates of perfusion-depleted tissues, possibly through enhancing local levels of growth factors.
开发并使用一种延迟的全层创面愈合模型，来检测单独或与富含血小板纤维蛋白凝胶联合使用的脂肪源性干细胞（ASCs）促进愈合的能力。
4 头猪接受电子束辐射照射背部皮肤表面。辐射后 5 周，从非照射区域采集皮下脂肪并进行处理以获得 ASCs。2 周后，在照射和非照射皮肤中制作 28 至 30 个 1.5cm²的全层 1.5cm² 创面。用生理盐水、生理盐水中的 ASCs、富含血小板的纤维蛋白凝胶（PRP）、PRP 中的 ASCs 或非自体绿色荧光蛋白标记的 ASCs 处理创面。
单次辐射剂量导致真皮微血管密度在 7 周内显著减少（75%）。生理盐水处理的照射和非照射皮肤之间的愈合率存在显著差异。与生理盐水相比，PRP 处理的 ASCs 处理的创面的愈合速度提高了 11.2%。增强作用取决于 ASCs 和 PRP 的组合，因为单独的 ASCs 或 PRP 都没有效果。
我们创建了一种模拟临床上与延迟创面愈合相关的晚期辐射效应的模型。使用该模型，我们表明 ASCs 和 PRP 的组合可提高灌注不足组织的愈合率，可能是通过增强局部生长因子水平。
