Janssens J P, Noto V, van Tendeloo G, Bonte J, de Loecker W
Department of Gynecological Oncology, University of Leuven, Belgium.
Anticancer Res. 1988 Jul-Aug;8(4):781-4.
To determine whether Müllerian Inhibiting Substance (MIS) is the factor responsible for the cytotoxic effects of testicular preparations on gynecological cancers, homogenates of bovine fetal testes were fractionated by Sephadex G 200 chromatography and their cytotoxic effects tested on a Müllerian-derived endometrial cancer cell line (AN3-CA), an ovarian carcinoma cell line (CAOV 3) and on a breast cancer cell line (MCF 7). No difference in cytotoxicity was found between Müllerian and non-Müllerian derived human cancer cells. Cytotoxicity and MIS activity were found in different fractions on Sephadex G 200 gel chromatography.
为了确定苗勒管抑制物质（MIS）是否是睾丸制剂对妇科癌症产生细胞毒性作用的原因，通过葡聚糖G 200色谱法对牛胎儿睾丸匀浆进行分级分离，并在源自苗勒管的子宫内膜癌细胞系（AN3-CA）、卵巢癌细胞系（CAOV 3）和乳腺癌细胞系（MCF 7）上测试其细胞毒性作用。在源自苗勒管和非苗勒管的人类癌细胞之间未发现细胞毒性差异。在葡聚糖G 200凝胶色谱的不同级分中发现了细胞毒性和MIS活性。
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