School of Integrative Engineering, College of Engineering, Chung-Ang University, Seoul 06974, Republic of Korea.
Biofabrication. 2019 Dec 23;12(1):015019. doi: 10.1088/1758-5090/ab5d3e.
Differentiation of stem cells into chondrocytes has been studied for the engineering of cartilage tissue. However, stem cells cultured two-dimensionally have limited ability to differentiate into chondrocytes, which led to the development of three-dimensional culture systems. A recently developed microtechnological method uses microwells as a tool to form uniformly sized spheroids. In this study, we fabricated an array (10 × 10) of goblet-shaped microwells based on polydimethylsiloxane for spheroid culture. A central processing unit (CPU) was used to form holes, and metallic beads were used to form hemispherical microwell geometry. The holes were filled with Pluronic F-127 to prevent cells from sinking through the holes and allowing the cells to form spheroids. Viability and chondrogenic differentiation of human adipose-derived stem cells were assessed. The fabrication method using a micro-pin mold and metallic beads is easy and cost-effective. Our three-dimensional spheroid culture system optimizes the efficient differentiation of cells and has various applications, such as drug delivery, cell therapy, and tissue engineering.
干细胞向软骨细胞的分化已被研究用于软骨组织的工程。然而，二维培养的干细胞向软骨细胞分化的能力有限，这导致了三维培养系统的发展。最近开发的一种微技术方法使用微井作为工具来形成均匀大小的球体。在这项研究中，我们基于聚二甲基硅氧烷制造了一种用于球体培养的十乘十的杯形微井阵列。中央处理器 (CPU) 用于形成孔，而金属珠用于形成半球形微井几何形状。孔内填充了 Pluronic F-127，以防止细胞沉入孔中，并允许细胞形成球体。评估了人脂肪来源干细胞的活力和软骨分化。使用微针模具和金属珠的制造方法简单且具有成本效益。我们的三维球体培养系统优化了细胞的高效分化，并具有多种应用，如药物输送、细胞治疗和组织工程。
