Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025, Hubei, China.
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, Jiangsu, China.
Arch Virol. 2020 Feb;165(2):487-490. doi: 10.1007/s00705-019-04486-5. Epub 2019 Nov 29.
A putative novel positive-sense (+) RNA virus was detected in isolate CF16158 of the fungus Fusarium graminearum, the causal agent of Fusarium head blight and crown rot in wheat in China. The full genome of this virus was sequenced and characterized. The complete cDNA sequence is 7,051 nt long and contains four open reading frames (ORFs). ORF2 is predicted to encode helicase (Hel) and RNA-dependent RNA polymerase (RdRp) domains that are conserved among the alphavirus-like viruses. Pairwise comparisons and phylogenetic analysis of the deduced amino acid sequences of Hel and RdRp indicated that this (+) RNA mycovirus is a novel member of a new, yet to be established family of alphavirus-like viruses. Therefore, we named this virus "Fusarium graminearum alphavirus-like virus 1" (FgALV1). This is the first report of a full-length genomic sequence of a putative alphavirus-like virus in F. graminearum.
在中国，一种假定的新型正链（+）RNA 病毒在真菌禾谷镰刀菌的分离株 CF16158 中被检测到，该真菌是小麦赤霉病和冠腐病的病原体。该病毒的全基因组已被测序和特征描述。该完整的 cDNA 序列长 7051 个核苷酸，包含四个开放阅读框（ORF）。ORF2 预测编码螺旋酶（Hel）和 RNA 依赖性 RNA 聚合酶（RdRp）结构域，这些结构域在类甲病毒病毒中保守。Hel 和 RdRp 推导氨基酸序列的成对比较和系统发育分析表明，这种（+）RNA 真菌病毒是一个尚未建立的新的类甲病毒病毒家族的新成员。因此，我们将这种病毒命名为“禾谷镰刀菌类甲病毒样病毒 1”（FgALV1）。这是在禾谷镰刀菌中发现的假定类甲病毒的全长基因组序列的首次报道。
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