Zhang Xiaoting, Gao Fei, Zhang Fang, Xie Yuan, Zhou Lin, Yuan Hongxia, Zhang Songbai, Li Honglian
College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, Henan, China.
Key Laboratory of Biopesticide and Chemical Biology Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
Arch Virol. 2018 Nov;163(11):3173-3175. doi: 10.1007/s00705-018-3970-z. Epub 2018 Jul 31.
A novel double-stranded RNA (dsRNA) mycovirus, named Fusarium pseudograminearum megabirnavirus 1 (FpgMBV1), was found in a hypovirulent strain FC136-2A of Fusarium pseudograminearum, the causal agent of wheat crown rot. The complete genome of FpgMBV1 comprised two dsRNA segments, L1-dsRNA (8951 bp) and L2-dsRNA (5337 bp). Both dsRNAs carry two open reading frames on the positive strand. L1-dsRNA potentially encodes a 131.3-kDa coat protein (CP) and a 126.3-kDa RNA-dependent RNA polymerase (RdRp), while L2-dsRNA encodes two putative proteins of 97.2 kDa and 30.6 kDa with unknown functions. As found in members of the family Megabirnaviridae, the 5'- and 3'-terminal sequences of L1-dsRNA and L2-dsRNA of FpgMBV1 are highly conserved. The identity between L1-dsRNA (1630 nt) and L2-dsRNA (1682 nt) is 88.95%, and the first 236 nt (nt 1-236) are exactly identical. The 3' UTRs of L1-dsRNA (155 nt) and L2-dsRNA (109 nt) are 66.67% identical, with identical terminal hexamer sequences (5'-AAAAGC-3'). Phylogenetic analyses based on the amino acid sequences of the RdRps indicated that FpgMBV1 is a new member of the family Megabirnaviridae.
在引起小麦根腐病的拟禾本科镰刀菌的低毒力菌株FC136-2A中发现了一种新型双链RNA（dsRNA）真菌病毒，命名为拟禾本科镰刀菌巨双RNA病毒1（FpgMBV1）。FpgMBV1的完整基因组由两个dsRNA片段组成，L1-dsRNA（8951 bp）和L2-dsRNA（5337 bp）。两条dsRNA在正链上都携带两个开放阅读框。L1-dsRNA可能编码一个131.3 kDa的衣壳蛋白（CP）和一个126.3 kDa的RNA依赖RNA聚合酶（RdRp），而L2-dsRNA编码两个功能未知的推定蛋白，分子量分别为97.2 kDa和30.6 kDa。正如在巨双RNA病毒科成员中所发现的，FpgMBV1的L1-dsRNA和L2-dsRNA的5'和3'末端序列高度保守。L1-dsRNA（1630 nt）和L2-dsRNA（1682 nt）之间的同一性为88.95%，前236 nt（nt 1-236）完全相同。L1-dsRNA（155 nt）和L2-dsRNA（109 nt）的3'非翻译区同一性为66.67%，具有相同的末端六聚体序列（5'-AAAAGC-3'）。基于RdRp氨基酸序列的系统发育分析表明，FpgMBV1是巨双RNA病毒科的一个新成员。
