Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of Korea.
Department of Horticulture, Kangwon National University, Chuncheon 24341, Republic of Korea.
Food Chem. 2020 Mar 30;309:125763. doi: 10.1016/j.foodchem.2019.125763. Epub 2019 Oct 25.
The study was conducted to confirm the effects of selenium biofortification on the bioactive compounds and antioxidant activity of wheat microgreen extract. The microgreens were cultivated in the DFT hydroponic system with different concentrations of Se (0 [control], 0.125, 0.25, 0.50, and 1.00 mg/L from sodium selenite) in a growth chamber by controlling temperature (25/20 °C, day/night), light (12 h light/dark; intensity 150 µmol‧m‧s with white fluorescence lamp), and humidity (60%) for 10 days. Se biofortification increased the germination rate and decreased microgreen length and yield. Chlorophyll and carotenoid levels increased in the Se-biofortified microgreen extract. Bioactive compounds such as phenolics, flavonoids, vitamin C, and anthocyanin significantly increased in 0.25-0.50 mg/L of Se-biofortified microgreen extracts. Antioxidant (ABTS, DPPH, NSA and SOD-like) activity also increased at moderate levels (0.25-0.50 mg/L) of Se biofortification. Therefore, Se biofortification may be useful for the industrial manufacture of new products from wheat microgreen extract.
本研究旨在确认硒生物强化对小麦苗提取物中生物活性化合物和抗氧化活性的影响。在生长室内，使用 DFT 水培系统，通过控制温度（25/20°C，日夜）、光照（12 小时光照/黑暗；强度为 150 µmol·m·s 的白色荧光灯）和湿度（60%），用亚硒酸钠将硒的浓度分别设置为 0（对照）、0.125、0.25、0.50 和 1.00 mg/L，在 10 天内培育不同浓度硒的小麦苗。硒的生物强化提高了发芽率，降低了麦苗的长度和产量。叶绿素和类胡萝卜素水平在富硒麦苗提取物中增加。生物活性化合物如酚类、类黄酮、维生素 C 和花青素在 0.25-0.50 mg/L 的富硒麦苗提取物中显著增加。抗氧化（ABTS、DPPH、NSA 和 SOD 样）活性也在适度的硒生物强化水平（0.25-0.50 mg/L）下增加。因此，硒的生物强化可能有助于从小麦苗提取物中工业生产新产品。
