Tokudome Yoshihiro
Faculty of Pharmacy and Pharmaceutical Sciences, Josai University.
Yakugaku Zasshi. 2019;139(12):1549-1551. doi: 10.1248/yakushi.19-00181-1.
Intercellular lipids in the stratum corneum (SC), including ceramides (CERs), cholesterol, and fatty acids, are important for maintaining the skin barrier function. CERs in the SC have vital roles in water retention and the barrier function. A decrease in intercellular lipids, however, reduces the skin barrier function. In this study, the ability of CER precursors to increase the level of CERs in the SC and improve the skin barrier function was examined. Glucosylceramide and sphingomyelin liposomes were used as CER precursors and prepared with a thin-film method. The particle diameter and surface potential of glucosylceramide liposomes were 120.0 nm and -20 mV, while those of sphingomyelin liposomes were 153.3 nm and -11.4 mV, respectively. Transmission electron microscopy images showed that both liposomes were closed vesicles having a lamellar structure. These liposomes were applied from the SC side of a three-dimensional cultured human epidermis model, and the level of CERs in the epidermis was measured by high-performance thin-layer chromatography. In this study, the application of glucosylceramide or sphingomyelin liposomes increased the amount of CERs. In addition, the precursors of CERs were effective in improving the skin barrier function.
角质层（SC）中的细胞间脂质，包括神经酰胺（CERs）、胆固醇和脂肪酸，对于维持皮肤屏障功能至关重要。SC中的CERs在保水和屏障功能方面发挥着重要作用。然而，细胞间脂质的减少会降低皮肤屏障功能。在本研究中，检测了CER前体增加SC中CERs水平并改善皮肤屏障功能的能力。葡萄糖神经酰胺和鞘磷脂脂质体用作CER前体，并采用薄膜法制备。葡萄糖神经酰胺脂质体的粒径和表面电位分别为120.0 nm和-20 mV，而鞘磷脂脂质体的粒径和表面电位分别为153.3 nm和-11.4 mV。透射电子显微镜图像显示，两种脂质体均为具有层状结构的封闭囊泡。将这些脂质体从三维培养的人表皮模型的SC侧应用，并通过高效薄层色谱法测量表皮中CERs的水平。在本研究中，葡萄糖神经酰胺或鞘磷脂脂质体的应用增加了CERs的量。此外，CERs的前体在改善皮肤屏障功能方面是有效的。
