Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China.
J Therm Biol. 2019 Dec;86:102436. doi: 10.1016/j.jtherbio.2019.102436. Epub 2019 Oct 10.
Demand for yellow catfish fry, an economically important farmed fish in China, has increased dramatically. Newly hatched larvae are highly sensitive to changes in environmental conditions, with water temperature (T) and dissolved oxygen (DO) being two important factors that affect their early development. We investigate optimal T (between 19.0 and 33.0 °C) and DO (between 2.0 and 12.0 mg L) concentrations on growth and antioxidant enzyme activity of newly hatched hybrid yellow catfish larvae (Tachysurus fulvidraco × Pseudobagrus vachellii) using a central composite design. We use a response surface method to optimize the response variables for survival (S) and growth, and the reduction of oxidative stress, over a 50-day experimental duration. T has a significant effect on specific growth rate (SGR), hepatic malondialdehyde (MDA) content, and superoxide dismutase (SOD) and catalase (CAT) activities (P < 0.05). DO concentration has a significant effect on SGR, S, hepatic MDA content, and SOD and CAT activities (P < 0.05). T and DO also have significant second order effects on SGR, S, SOD, and CAT activities (P < 0.05). Increased DO at low T stimulates SOD and CAT activities and alleviates oxidative damage. Adjusted R values for SGR, S, CAT, SOD, and MDA models are 0.734, 0.937, 0.916, 0.894 and 0.826, respectively. A combination of 26.8 °C and 7.3 mg L represents optimal rearing conditions, in that larval growth and antioxidant ability is improved. Results show that T and DO during larviculture of yellow catfish have important implications for aquaculture.
黄颡鱼鱼苗的需求在中国急剧增加，这种鱼苗是一种具有重要经济价值的养殖鱼类。刚孵化的幼鱼对环境条件的变化非常敏感，而水温（T）和溶解氧（DO）是影响其早期发育的两个重要因素。我们采用中心复合设计研究了水温（19.0 到 33.0°C）和 DO（2.0 到 12.0mg/L）浓度对杂交黄颡鱼（Tachysurus fulvidraco × Pseudobagrus vachellii）刚孵化幼鱼生长和抗氧化酶活性的影响。我们使用响应面法来优化 50 天实验期间的存活率（S）、生长和氧化应激减少的响应变量。T 对特定生长率（SGR）、肝丙二醛（MDA）含量以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性有显著影响（P<0.05）。DO 浓度对 SGR、S、肝 MDA 含量以及 SOD 和 CAT 活性有显著影响（P<0.05）。T 和 DO 对 SGR、S、SOD 和 CAT 活性也有显著的二阶效应（P<0.05）。在低 T 时增加 DO 会刺激 SOD 和 CAT 活性并减轻氧化损伤。SGR、S、CAT、SOD 和 MDA 模型的调整 R 值分别为 0.734、0.937、0.916、0.894 和 0.826。26.8°C 和 7.3mg/L 的组合代表了最佳的养殖条件，可提高幼鱼的生长和抗氧化能力。结果表明，黄颡鱼鱼苗养殖过程中的 T 和 DO 对水产养殖具有重要意义。
