Department for Infectious Diseases, University of Heidelberg, Heidelberg, Germany.
J Med Microbiol. 2020 Jan;69(1):46-48. doi: 10.1099/jmm.0.001104.
The question of whether a single day of incubation is sufficient for urine cultures has been a matter of debate. The aim of this study was to investigate the potential benefit of prolonged incubation for initially culture-negative urines. Eight hundred and twelve urine specimens with no growth after incubation for 20 h were incubated for an additional 20 h to detect slower growing uropathogenic organisms. This study included a considerable number of urine cultures from immunocompromised and/or kidney-transplanted patients. For 99.9 % of the specimens, there was no difference in the interpretation of results. Twenty hours of incubation did not have any negative effect on the detection of uropathogens.
关于尿液培养是否需要一天的孵育时间一直存在争议。本研究旨在探讨延长孵育时间对初始培养阴性尿液的潜在益处。对 812 份孵育 20 小时后无生长的尿液标本进行了额外 20 小时的孵育，以检测生长缓慢的尿路病原体。本研究包括相当数量的免疫功能低下和/或肾移植患者的尿液培养物。对于 99.9%的标本，结果的解释没有差异。20 小时的孵育对尿路病原体的检测没有任何负面影响。
