Academy for Advanced Interdisciplinary Studies (AAIS), Peking University, Beijing, China.
Biomedical Pioneering Innovation Center (BIOPIC), and State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, China.
PLoS One. 2019 Dec 2;14(12):e0225466. doi: 10.1371/journal.pone.0225466. eCollection 2019.
The HeLa cells are the earliest and mostly used laboratory human cells for biomedical particularly cancer research. They were derived from a patient's cervical cancerous tissue, and are known for their heterogeneous cellular origin and variable genomic landscapes. Single-cell sequencing techniques with faithful linear and uniformly amplified genomes (DNA) and transcriptomes (RNA) may facilitate the study of cellular differences at the individual cell level. In this work, we have performed single-cell DNA and RNA sequencing with HeLa-CCL2 cells to study their heterogeneity. We have studied the complexity of copy number variations (CNVs) of HeLa-CCL2 genome at the single cell level, and revealed the transcriptomic heterogeneity of HeLa-CCL2. We also analyzed the relationship between genome and transcriptome at the single-cell level, and found overall correlation between CNV and transcriptome expression patterns. Finally, we concluded that although single-cell sequencing techniques are applicable to study heterogeneous cells such as HeLa-CCL2, the data analyses need to be more careful and well controlled.
海拉细胞系是最早也是最常用于生物医学，特别是癌症研究的实验室人类细胞系。它们源自一位宫颈癌患者的组织，以其异质的细胞起源和不同的基因组景观而闻名。具有忠实线性和均匀扩增基因组（DNA）和转录组（RNA）的单细胞测序技术可能有助于在单细胞水平上研究细胞差异。在这项工作中，我们对 HeLa-CCL2 细胞进行了单细胞 DNA 和 RNA 测序，以研究其异质性。我们研究了 HeLa-CCL2 基因组在单细胞水平上的拷贝数变异（CNV）的复杂性，并揭示了 HeLa-CCL2 的转录组异质性。我们还分析了单细胞水平上基因组和转录组之间的关系，并发现 CNV 和转录组表达模式之间存在整体相关性。最后，我们得出结论，尽管单细胞测序技术适用于研究海拉-CCL2 等异质细胞，但数据分析需要更加小心和严格控制。
