Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, Republic of Korea.
Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, Republic of Korea; Institute of Convergence Bio-Health, Dong-A University, Busan, 604-714, Republic of Korea.
Food Chem Toxicol. 2020 Feb;136:111011. doi: 10.1016/j.fct.2019.111011. Epub 2019 Nov 30.
Neurite outgrowth is important process in synaptic formation and neuronal development. Many previous studies reported that natural compounds as well as neurotrophins induce neurite outgrowth through various signaling pathways. In this study, we tested the effect of cryptotanshinone (CPT), a constituent of Salvia miltiorrhiza Bunge, on neurite outgrowth using neuro2a cell line, a mouse neuroblastoma cell line. And then, we examined the effect of CPT on learning and memory. We first found that CPT facilitated neurite outgrowth in a concentration-dependent manner. Although CPT induced MTT reduction, CPT did not induce LDH release. Moreover, CPT suppressed cell proliferation. CPT increased ERK1/2 phosphorylation and ERK1/2 inhibitor blocked CPT-facilitated neurite outgrowth. CPT also enhanced learning and memory without affecting basal sensory conditions and increased ERK1/2 phosphorylation in the hippocampus in a dose-dependent manner. These results demonstrate that CPT facilitates neurite outgrowth and enhances learning and memory, which may be mediated by facilitating ERK1/2 signal.
神经突生长是突触形成和神经元发育的重要过程。许多先前的研究报告称，天然化合物和神经营养因子通过各种信号通路诱导神经突生长。在这项研究中，我们使用神经母细胞瘤细胞系 Neuro2a 细胞系测试了隐丹参酮（CPT），丹参的一种成分对神经突生长的影响，然后，我们研究了 CPT 对学习和记忆的影响。我们首先发现 CPT 以浓度依赖的方式促进神经突生长。尽管 CPT 诱导 MTT 减少，但 CPT 不会诱导 LDH 释放。此外，CPT 抑制细胞增殖。CPT 增加 ERK1/2 磷酸化，ERK1/2 抑制剂阻断 CPT 促进的神经突生长。CPT 还增强了学习和记忆，而不影响基础感觉条件，并以剂量依赖的方式增加海马中的 ERK1/2 磷酸化。这些结果表明 CPT 促进神经突生长并增强学习和记忆，这可能是通过促进 ERK1/2 信号介导的。
