Clinical Microbiology Laboratory, Saint-Joseph Hospital, Paris, France.
Vascular Medicine and Phlebology Service, Saint-Joseph Hospital, Paris, France.
J Microbiol Methods. 2020 Jan;168:105796. doi: 10.1016/j.mimet.2019.105796. Epub 2019 Nov 29.
Unyvero i60 ITI multiplex PCR (mPCR) may identify a large panel of bacteria and antibiotic resistance genes. In this study, we compared results obtained by mPCR to standard bacteriology in chronic leg ulcer (CLU) infections.
A prospective study, part of the interventional-blinded randomized study "ulcerinfecte" (NCT02889926), was conducted at Saint Joseph Hospital in Paris. Fifty patients with a suspicion of infected CLU were included between February 2017 and September 2018. Conventional bacteriology and mPCR were performed simultaneously on deep skin biopsies.
Staphylococcus aureus and Pseudomonas aeruginosa were the most detected pathogens. Regarding the global sensitivity, mPCR is not overcome to the standard culture. Anaerobes and slow growing bacteria were detected with a higher sensitivity rate by mPCR than standard culture.
Unyvero i60 ITI multiplex PCR detected rapidly pathogenic bacteria in infected CLU especially anaerobes and slow growing bacteria and was particularly effective for patients previously treated with antibiotics.
Unyvero i60 ITI 多重 PCR（mPCR）可鉴定出大量细菌和抗生素耐药基因。本研究比较了 mPCR 与慢性腿部溃疡（CLU）感染中标准细菌学的结果。
这是一项前瞻性研究，是“ulcerinfecte”（NCT02889926）干预性盲法随机研究的一部分，在巴黎圣约瑟夫医院进行。2017 年 2 月至 2018 年 9 月期间，共纳入 50 例疑似感染 CLU 的患者。对深部皮肤活检标本同时进行常规细菌学和 mPCR 检测。
金黄色葡萄球菌和铜绿假单胞菌是最常检测到的病原体。关于总体敏感性，mPCR 并未优于标准培养。mPCR 比标准培养检测到更多的厌氧菌和生长缓慢的细菌。
Unyvero i60 ITI 多重 PCR 可快速检测出感染性 CLU 中的致病细菌，特别是厌氧菌和生长缓慢的细菌，对于先前接受过抗生素治疗的患者尤其有效。
