从人诱导多能干细胞（MHHi006-A-4）生成NKX2.1 - p63双转基因敲入报告细胞系。
Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4).
作者信息
Drick Nora, Sahabian Anais, Pongpamorn Praeploy, Merkert Sylvia, Göhring Gudrun, Welte Tobias, Martin Ulrich, Olmer Ruth
机构信息
Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany; Department of Pneumology and German Center for Lung Research, Hannover Medical School, 30625 Hannover, Germany.
Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany.
出版信息
Stem Cell Res. 2020 Jan;42:101659. doi: 10.1016/j.scr.2019.101659. Epub 2019 Nov 19.
Tumor protein p63 (p63) encodes for a transcription factor of the p53 family and is a marker for respiratory basal cells. Based on a NKX2.1 knock-in reporter cell line from human induced pluripotent stem cells (hiPSCs) (MHHi06-A-2) we established a NKX2.1/p63 double transgenic knock-in reporter cell line using TALEN technology. The reporter enables the optimization and monitoring of hiPSC differentiation towards NKX2.1/p63 double positive cells as well as enrichment for single or double positive cells.
肿瘤蛋白p63（p63）编码p53家族的一种转录因子，是呼吸道基底细胞的标志物。基于来自人诱导多能干细胞（hiPSC）的NKX2.1敲入报告细胞系（MHHi06-A-2），我们使用TALEN技术建立了NKX2.1/p63双转基因敲入报告细胞系。该报告基因能够优化和监测hiPSC向NKX2.1/p63双阳性细胞的分化，以及对单阳性或双阳性细胞进行富集。
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