Mammeri Mohamed, Cartou Lara, Chevillot Aurélie, Thomas Myriam, Julien Christine, Vallée Isabelle, Polack Bruno, Follet Jérôme, Adjou Karim Tarik
UMR BIPAR, Ecole Nationale Vétérinaire d'Alfort, ANSES, INRA, Université Paris-Est, Maisons-Alfort F-94700, France; Phileo Lesaffre Animal Care, 137 rue Gabriel Péri, 59 700 Marcq-en-Barœul, France.
UMR BIPAR, Ecole Nationale Vétérinaire d'Alfort, ANSES, INRA, Université Paris-Est, Maisons-Alfort F-94700, France.
Vet Parasitol Reg Stud Reports. 2019 Dec;18:100355. doi: 10.1016/j.vprsr.2019.100355. Epub 2019 Nov 23.
To date, no information is available about the presence of Cryptosporidium spp. in French sheep, nor their potential role as zoonotic reservoirs. A total of 23 fecal samples were collected from diarrheic lambs (<11 days old) from seven randomly selected farms. Cryptosporidium-oocysts were detected microscopically with Direct Immunofluorescence Assays (DFA) in 23/23 (100%) of fecal samples. PCR-RFLP of the 18S rRNA gene was used to determine species in all samples, and only Cryptosporidium parvum was identified. Isolates were subtyped by sequencing the 60 kDa glycoprotein (gp60) gene. Two zoonotic subtypes within the IIa subtype family were identified, including IIaA15G2R1 (22/23) and IIaA16G3R1 (1/23). This study reports for the first time the identification and genotyping of zoonotic C. parvum subtypes from lambs in France. Sheep could thus play an important role as potential reservoirs for this zoonotic protist.
迄今为止，尚无关于法国绵羊体内隐孢子虫属存在情况的信息，也不清楚它们作为人畜共患病原体宿主的潜在作用。从七个随机选择的农场中，收集了23份来自腹泻羔羊（小于11日龄）的粪便样本。通过直接免疫荧光法（DFA）在显微镜下检测到23份粪便样本中均有隐孢子虫卵囊（检出率100%）。使用18S rRNA基因的PCR-RFLP方法确定所有样本中的虫种，结果仅鉴定出微小隐孢子虫。通过对60 kDa糖蛋白（gp60）基因进行测序对分离株进行亚型分析。在IIa亚型家族中鉴定出两种人畜共患亚型，包括IIaA15G2R1（22/23）和IIaA16G3R1（1/23）。本研究首次报道了法国羔羊体内人畜共患微小隐孢子虫亚型的鉴定和基因分型。因此，绵羊可能作为这种人畜共患原生生物的潜在宿主发挥重要作用。
