神经和肌肉可溶性因子对大鼠离体肌细胞膜中α-银环蛇毒素结合位点的影响。
Effect of soluble factors from nerve and muscle on alpha-bungarotoxin binding sites in isolated sarcolemmal membranes of the rat.
作者信息
Harborne A J, Smith M E
机构信息
Department of Physiology, Medical School, University of Birmingham.
出版信息
Br J Pharmacol. 1988 Jul;94(3):968-76. doi: 10.1111/j.1476-5381.1988.tb11611.x.
Abstract
- Sarcolemmal membranes were isolated from normal and from denervated muscles and the specific binding of [125I]-alpha-bungarotoxin to the membranes was determined. 2. Cytosol prepared from either slow (soleus) or fast (extensor digitorum longus) muscle increased the toxin binding. Similar effects were seen with cytosol from muscles which had been denervated 5 to 7 days previously and with cytosol from sciatic nerve. A purified preparation of a bacterial phospholipase C also produced this effect. 3. Bathing medium in which normal or denervated muscles had been incubated, increased the toxin binding to normal membranes, but the normal bathing medium decreased the binding to membranes prepared from denervated muscles. 4. The possibility that pre-existing latent acetylcholine receptors can be activated by intracellular factors released from muscles is discussed.
摘要
- 从正常肌肉和失神经支配的肌肉中分离出肌膜，并测定[125I] -α-银环蛇毒素与这些膜的特异性结合。2. 从慢肌（比目鱼肌）或快肌（趾长伸肌）制备的胞质溶胶增加了毒素结合。来自5至7天前已失神经支配的肌肉的胞质溶胶以及来自坐骨神经的胞质溶胶也观察到类似效果。一种纯化的细菌磷脂酶C制剂也产生了这种效果。3. 曾用于孵育正常或失神经支配肌肉的孵育液增加了毒素与正常膜的结合，但正常孵育液降低了毒素与从失神经支配肌肉制备的膜的结合。4. 讨论了预先存在的潜在乙酰胆碱受体可被肌肉释放的细胞内因子激活的可能性。
相似文献
1
Effect of soluble factors from nerve and muscle on alpha-bungarotoxin binding sites in isolated sarcolemmal membranes of the rat.神经和肌肉可溶性因子对大鼠离体肌细胞膜中α-银环蛇毒素结合位点的影响。
Br J Pharmacol. 1988 Jul;94(3):968-76. doi: 10.1111/j.1476-5381.1988.tb11611.x.
2
Effect of phospholipase C on the binding of alpha-bungarotoxin to isolated plasma membranes of rat skeletal muscle.磷脂酶C对α-银环蛇毒素与大鼠骨骼肌分离质膜结合的影响。
Neurosci Lett. 1986 Dec 12;72(2):135-40. doi: 10.1016/0304-3940(86)90068-6.
3
Decreased acetylcholine receptor content in denervated skeletal muscles infused with nerve extract.注入神经提取物的失神经支配骨骼肌中乙酰胆碱受体含量降低。
J Neurosci Res. 1986;16(3):517-25. doi: 10.1002/jnr.490160307.
4
Congruity of acetylcholine receptor, acetylcholinesterase, and Dolichos biflorus lectin binding glycoprotein in postsynaptic-like sarcolemmal specializations in noninnervated regenerating rat muscles.未受神经支配的再生大鼠肌肉中突触后样肌膜特化结构中乙酰胆碱受体、乙酰胆碱酯酶和双花扁豆凝集素结合糖蛋白的一致性。
J Neurosci Res. 1993 Jan;34(1):67-78. doi: 10.1002/jnr.490340108.
5
Effect of nerve extract on number of acetylcholine receptors in denervated muscles of rats.神经提取物对大鼠失神经肌肉中乙酰胆碱受体数量的影响。
Exp Neurol. 1984 Jan;83(1):108-17. doi: 10.1016/0014-4886(84)90050-5.
6
Exposure of receptor sites on rat muscle membranes by a neurally released factor.神经释放因子使大鼠肌肉膜上的受体位点暴露。
Neurosci Lett. 1987 Jun 26;77(3):283-6. doi: 10.1016/0304-3940(87)90513-1.
7
Appearance of new acetylcholine receptors on the baby chick biventer cervicis and denervated rat diaphragm muscles after blockade with alpha-bungarotoxin.用α-银环蛇毒素阻断后，雏鸡颈二腹肌和去神经大鼠膈肌上新乙酰胆碱受体的出现。
J Physiol. 1977 Jun;268(2):449-65. doi: 10.1113/jphysiol.1977.sp011865.
8
Autoradiographic changes of 3H-alpha-bungarotoxin binding in rat hind limb muscles after a cryoinjury of the sciatic nerve and in acrylamide intoxication.坐骨神经冷冻损伤及丙烯酰胺中毒后大鼠后肢肌肉中3H-α-银环蛇毒素结合的放射自显影变化
Acta Physiol Scand. 1985 May;124(1):117-20. doi: 10.1111/j.1748-1716.1985.tb07639.x.
9
Effect of denervation on sarcolemmal proteins and glycoproteins of fast and slow mammalian skeletal muscle.
Exp Neurol. 1986 Feb;91(2):229-45. doi: 10.1016/0014-4886(86)90064-6.
10
alpha-bungarotoxin binding sites (acetylcholine receptors) in denervated mammalian sarcolemma.去神经支配的哺乳动物质膜中的α-银环蛇毒素结合位点（乙酰胆碱受体）。
J Supramol Struct. 1979;12(3):321-34. doi: 10.1002/jss.400120305.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY.用于电子显微镜检查的塑料包埋混合物
Stain Technol. 1964 Mar;39:111-4.
3
ISOLATION OF NEUROTOXINS FROM THE VENOM OF BUNGARUS MULTICINCTUS AND THEIR MODES OF NEUROMUSCULAR BLOCKING ACTION.从银环蛇毒液中分离神经毒素及其神经肌肉阻断作用方式
Arch Int Pharmacodyn Ther. 1963 Jul 1;144:241-57.
4
Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.营养不良性肌肉的生物化学。线粒体琥珀酸 - 四氮唑还原酶和三磷酸腺苷酶。
Biochem J. 1961 Sep;80(3):649-54. doi: 10.1042/bj0800649.
5
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
6
Lactic dehydrogenase activity in blood.血液中的乳酸脱氢酶活性。
Proc Soc Exp Biol Med. 1955 Oct;90(1):210-3. doi: 10.3181/00379727-90-21985.
7
Unmasking of cryptic binding sites for alpha -bungarotoxin in membrane fractions from chick retina.鸡视网膜膜组分中α-银环蛇毒素隐蔽结合位点的揭示
Biochem Biophys Res Commun. 1981 Oct 30;102(4):1385-92. doi: 10.1016/s0006-291x(81)80165-9.
8
The potentiating action of acetylcholine on the chemosensitivity of denervated skeletal muscle.
Neuroscience. 1982;7(12):3163-72. doi: 10.1016/0306-4522(82)90238-x.
9
Release of hydrolases and acetylcholine sensitivity in rat skeletal muscle.大鼠骨骼肌中水解酶的释放及乙酰胆碱敏感性
J Physiol. 1984 Jul;352:385-93. doi: 10.1113/jphysiol.1984.sp015298.
10
Heterogeneity of the calcium-dependent phosphatidylinositol phosphodiesterase in rat brain.大鼠脑中钙依赖性磷脂酰肌醇磷酸二酯酶的异质性
Biochem J. 1982 Aug 1;205(2):437-42. doi: 10.1042/bj2050437.
Zotero 插件