Faculty of Education, Kyoto University of Education, Kyoto, Japan.
Methods Mol Biol. 2020;2094:91-99. doi: 10.1007/978-1-0716-0183-9_10.
The organization of the root apical meristem (RAM) provides insights into the evolution of roots in vascular plants. The RAM of seed plants has a quiescent center (QC), in which the cells divide infrequently and function to maintain neighboring stem cells. However, the existence of a QC and the mechanisms of RAM maintenance in non-seed plants are poorly understood. We analyzed the RAM organization of lycophytes focusing on cell division activity using the EdU labeling method and showed that the RAM of Lycopodium species has a region with a very low cell division frequency, which was named the QC-like region. Here, we describe an in situ EdU labeling method for the RAM of growing roots in nature.
根顶端分生组织（RAM）的组织为研究维管植物根的进化提供了线索。种子植物的 RAM 有一个静止中心（QC），其中的细胞分裂不频繁，其功能是维持邻近的茎细胞。然而，QC 的存在以及非种子植物 RAM 维持的机制还知之甚少。我们通过 EdU 标记法分析了石松类植物的 RAM 组织，重点研究了细胞分裂活性，并表明石松属植物的 RAM 具有一个细胞分裂频率非常低的区域，我们将其命名为 QC 样区域。在这里，我们描述了一种在自然生长的根的 RAM 中进行原位 EdU 标记的方法。
