Tedesco Maybell, Chain Marcelo Carvalho, Felippe Wilson Tadeu, Alves Ana Maria Hecke, Garcia Lucas da Fonseca Roberti, Bortoluzzi Eduardo Antunes, Cordeiro Mabel Rodriguez, Teixeira Cleonice Silveira
Department of Dentistry, Endodontics Division, Health Sciences Center, UFSC - Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Department of Morphological Sciences, UFSC - Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Braz Dent J. 2019 Nov-Dec;30(6):555-562. doi: 10.1590/0103-6440201902766.
This study correlated the bond strength (BS) and dentin penetration of different sealers by push-out test and Confocal Laser Scanning Microscopy (CLSM) analysis. Forty-five root canals were prepared according to the crown-down technique and filled with gutta-percha associated to the following sealers (n=15): Endofill, AH Plus and MTA Fillapex. Five canals of each group were filled with the sealers added with 0.1% Rhodamine B dye. Next, the specimens were transversely sectioned and submitted to the push-out test (n=10) and CLSM analysis (n=5). The BS data showed the following means (MPa) and standard deviation: AH Plus (4.17±1.86); MTA Fillapex (3.13±1.96) and Endofill (2.10±1.03). Statistical analysis (two-way ANOVA, α=0.05) showed significant difference among sealers (p<0.001) and root canal thirds (p<0.001). The BS results of Endofill and MTA Fillapex were statistically similar (p>0.05), however, they were statistically different from AH Plus (p<0.001). The regional analysis of BS showed similarity between middle and apical thirds (p>0.05), and both were different from coronal portion (p<0.001). CLSM analysis verified tags formation in all groups and higher penetration of the specimens filled with AH Plus (p<0.05). The Kendall test (correlation between BS to dentin and sealer penetration into dentinal tubules) and the Pearson test (between failures pattern and sealer penetration into dentinal tubules) did not show correlation between the variables evaluated for all the tested sealers (p>0.05). AH Plus group had higher BS to dentin, and deeper tags formation than the other sealers. There was no significant correlation between BS and intratubular penetration of the tested sealers.
本研究通过推出试验和共聚焦激光扫描显微镜（CLSM）分析，对不同封闭剂的粘结强度（BS）和牙本质渗透情况进行了相关性研究。按照冠向下技术预备45个根管，并用牙胶尖充填，分别联合使用以下封闭剂（n = 15）：Endofill、AH Plus和MTA Fillapex。每组5个根管的封闭剂中添加0.1%的罗丹明B染料。接下来，将标本横向切片，进行推出试验（n = 10）和CLSM分析（n = 5）。BS数据显示出以下均值（MPa）和标准差：AH Plus（4.17±1.86）；MTA Fillapex（3.13±1.96）和Endofill（2.10±1.03）。统计分析（双向方差分析，α = 0.05）显示，封闭剂之间（p < 0.001）和根管的三个部分之间（p < 0.001）存在显著差异。Endofill和MTA Fillapex的BS结果在统计学上相似（p > 0.05），然而，它们与AH Plus在统计学上存在差异（p < 0.001）。BS的区域分析显示，中部和根尖三分之一之间相似（p > 0.05），且两者均与冠部不同（p < 0.001）。CLSM分析证实所有组均有标签形成，且充填AH Plus的标本有更高的渗透（p < 0.05）。肯德尔检验（BS与牙本质以及封闭剂渗入牙本质小管之间的相关性）和皮尔逊检验（失败模式与封闭剂渗入牙本质小管之间的相关性）显示，对于所有测试的封闭剂，所评估的变量之间均无相关性（p > 0.05）。AH Plus组与牙本质的BS更高，且标签形成比其他封闭剂更深。测试封闭剂的BS与管内渗透之间无显著相关性。
