将一种真核转录抑制剂转化为激活剂。

Converting a eukaryotic transcriptional inhibitor into an activator.

作者信息

Ma J, Ptashne M

机构信息

Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

出版信息

Cell. 1988 Nov 4;55(3):443-6. doi: 10.1016/0092-8674(88)90030-x.

DOI:10.1016/0092-8674(88)90030-x

PMID:3180218

Abstract

GAL80, an inhibitor of the yeast transcriptional activator GAL4, is converted into an activator by inserting an acidic activating sequence into it. This hybrid activator does not bind to DNA directly, but is brought to DNA by interacting with a derivative of GAL4 that interacts with both DNA and GAL80.

摘要

GAL80是酵母转录激活因子GAL4的一种抑制剂，通过向其中插入一段酸性激活序列可将其转化为激活因子。这种杂合激活因子并不直接与DNA结合，而是通过与GAL4的一种衍生物相互作用被带到DNA上，该GAL4衍生物既能与DNA相互作用，也能与GAL80相互作用。