Morris C D, Srihongse S
Am J Trop Med Hyg. 1978 Nov;27(6):1246-50. doi: 10.4269/ajtmh.1978.27.1246.
Evidence for and against the hypothesis of transovarial transmission by Culiseta melanura was obtained during an ongoing eastern equine encephalomyelitis (EEE) surveillance and control program. Evidence inconsistent with transovarial transmission included failure to isolated virus from 1,047 larvae, from 2,140 first-brood adults, or from 8,919 males collected at the same time as 3,977 nonblooded females which yielded 12 EEE isolates. Evidence supporting the hypothesis was the isolation of virus from both blooded and nonblooded adults simultaneously and also from a population with a parity rate so low that the infection rate for parous specimens would have been 1:8. Two alternative hypotheses which assume transovarial transmission are advanced to explain these results, but they seem so unlikely that the data are interpreted as opposing the concept.
在一项正在进行的东部马脑炎（EEE）监测与控制项目中，获取了支持和反对黑尾库蚊经卵传递假说的证据。与经卵传递不一致的证据包括：未能从1047只幼虫、2140只第一代成虫或与3977只未吸血雌蚊同时采集的8919只雄蚊中分离出病毒，而这些未吸血雌蚊中有12株EEE病毒分离株。支持该假说的证据是，同时从吸血和未吸血成虫中分离出了病毒，并且还从一个产卵率极低的种群中分离出了病毒，以至于经产标本的感染率本应为1:8。提出了两个假定经卵传递的替代假说，以解释这些结果，但它们似乎极不可能成立，因此这些数据被解释为反对这一概念。
