Şebinkarahisar School of Applied Sciences, Department of Food Technology, Giresun University, 28400, Giresun, Turkey.
Faculty of Science and Art, Department of Biology, Giresun University, 28049, Giresun, Turkey.
Environ Sci Pollut Res Int. 2020 Jan;27(1):657-667. doi: 10.1007/s11356-019-06920-2. Epub 2019 Dec 5.
Excessive copper (Cu) exposure ultimately results in toxicosis in all organisms. The protective potential of resveratrol compound against the CuCl toxicity was evaluated in Allium cepa L. root tip cells. For this aim, A. cepa bulbs were divided into six groups and the groups were treated with tap water, 400 mg/L resveratrol, 800 mg/L resveratrol, 20 μM CuCl, 400 mg/L resveratrol + 20 μM CuCl, and 800 mg/L resveratrol + 20 μM CuCl solutions, respectively for 72 h. The first group irrigated with tap water was accepted as control. All groups were screened for the germination percentage, root elongation, total bulb weight gain, micronucleus (MN) frequency, mitotic index (MI), chromosomal aberrations, and anatomical changes. Furthermore, superoxide dismutase (SOD) and catalase (CAT) activities as well as malondialdehyde (MDA) level as an indicator of lipid peroxidation were investigated. It was found that CuCl exposure alone triggered a definite negative effects on all parameters examined. On the other hand, the groups treated with resveratrol did not have statistically different values compared to the control group. Resveratrol doses induced a remarkable recovery in growth parameters including germination percentage, root elongation, and total bulb weight gain when applied with CuCl. In these groups, MN frequency, chromosomal aberrations, and anatomical abnormalities were alleviated, whereas the MI levels increased significantly. Moreover, lipid peroxidation level and antioxidant enzyme activities showed a marked amelioration when resveratrol and CuCl were applied together. Copper compounds have become common pollutants due to their direct uses as pesticides in agricultural areas as well as their spreading to natural areas from industrial fields. The study clearly demonstrated the therapeutic potential of resveratrol against the harmful effects of CuCl exposure in Allium roots. So, resveratrol could be considered as a plant-derived restorative agent for the reduction of the risks from the other copper compounds.
过量的铜（Cu）暴露最终会导致所有生物体中毒。本研究旨在评估白藜芦醇化合物对 CuCl 毒性的保护潜力，采用洋葱根尖细胞作为实验材料。为此，将洋葱鳞茎分为六组，分别用自来水、400 mg/L 白藜芦醇、800 mg/L 白藜芦醇、20 μM CuCl、400 mg/L 白藜芦醇+20 μM CuCl 和 800 mg/L 白藜芦醇+20 μM CuCl 溶液处理 72 h。用自来水处理的第一组作为对照。检测各组的发芽率、根伸长、总鳞茎增重、微核（MN）频率、有丝分裂指数（MI）、染色体畸变和解剖变化。此外，还研究了超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及丙二醛（MDA）水平作为脂质过氧化的指标。结果表明，单独暴露于 CuCl 会对所有检测参数产生明显的负面影响。另一方面，用白藜芦醇处理的各组与对照组相比，其数值没有统计学差异。当与 CuCl 一起应用时，白藜芦醇剂量会显著恢复生长参数，包括发芽率、根伸长和总鳞茎增重。在这些组中，MN 频率、染色体畸变和解剖异常得到缓解，而 MI 水平显著增加。此外，当同时应用白藜芦醇和 CuCl 时，脂质过氧化水平和抗氧化酶活性明显改善。由于铜化合物在农业领域直接用作农药以及从工业领域扩散到自然环境，因此它们已成为常见的污染物。该研究清楚地证明了白藜芦醇对洋葱根尖中 CuCl 暴露的有害影响的治疗潜力。因此，白藜芦醇可以被认为是一种植物衍生的修复剂，可降低其他铜化合物的风险。
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