胚胎肢芽间充质细胞和肌肉来源的成纤维细胞对转化生长因子-β的趋化反应。

Chemotactic response of embryonic limb bud mesenchymal cells and muscle-derived fibroblasts to transforming growth factor-beta.

作者信息

Lucas P A, Caplan A I

机构信息

Department of Biology, Case Western Reserve University, Cleveland, OH 44106.

出版信息

Connect Tissue Res. 1988;18(1):1-7. doi: 10.3109/03008208809019068.

DOI:10.3109/03008208809019068

PMID:3180812

Abstract

Transforming growth factor beta (TGF-beta) was tested for its ability to stimulate a chemotactic response in Stage 24 embryonic chick limb bud mesenchymal cells and muscle-derived fibroblasts. TGF-beta stimulated dose-dependent chemotaxis in both cell populations. Maximal chemotaxis was achieved with a concentration of 5 ng/ml for limb bud cells and as low as 15 pg/ml for muscle-derived fibroblasts. TGF-beta was not chemokinetic at these levels. Several other proteins found in bone, namely fibronectin, type I collagen, and osteonectin, were not chemotactic. However, both Bone Gla-protein and basic-FGF were found to be chemotactic but less effective than TGF-beta. Comparison with extracts of adult bone indicates that while TGF-beta is a potent chemoattractant, it does not account for all the chemotactic activity found in adult bone.

摘要

检测了转化生长因子β（TGF-β）刺激第24阶段鸡胚肢芽间充质细胞和肌肉来源的成纤维细胞产生趋化反应的能力。TGF-β在这两种细胞群体中均刺激剂量依赖性趋化作用。肢芽细胞在浓度为5 ng/ml时达到最大趋化作用，而肌肉来源的成纤维细胞在低至15 pg/ml时达到最大趋化作用。在这些水平下，TGF-β没有化学促动作用。在骨中发现的其他几种蛋白质，即纤连蛋白、I型胶原蛋白和骨粘连蛋白，没有趋化作用。然而，骨钙蛋白和碱性成纤维细胞生长因子（basic-FGF）均被发现具有趋化作用，但比TGF-β的效果要差。与成年骨提取物的比较表明，虽然TGF-β是一种有效的化学引诱剂，但它并不能解释成年骨中发现的所有趋化活性。