转化生长因子β对人成纤维细胞趋化性迁移的刺激作用。
Stimulation of the chemotactic migration of human fibroblasts by transforming growth factor beta.
作者信息
Postlethwaite A E, Keski-Oja J, Moses H L, Kang A H
出版信息
J Exp Med. 1987 Jan 1;165(1):251-6. doi: 10.1084/jem.165.1.251.
Abstract
Transforming growth factor beta (TGF-beta) is a potent chemoattractant in vitro for human dermal fibroblasts. Intact disulfide and perhaps the dimeric structure of TGF-beta is essential for its ability to stimulate chemotactic migration of fibroblasts, since reduction with 2-ME results in a marked loss of its potency as a chemoattractant. Although epidermal growth factor (EGF) appears to be capable of modulating some effects of TGF-beta, it does not alter the chemotactic response of fibroblasts to TGF-beta. Specific polyvalent rabbit antibodies to homogeneously pure TGF-beta block its chemotactic activity but has no effect on the other chemoattractants tested (platelet-derived growth factor, fibronectin, and denatured type I collagen). Since TGF-beta is secreted by a variety of neoplastic and normal cells including platelets, monocytes/macrophages, and lymphocytes, it may play a critical role in vivo in embryogenesis, host response to tumors, and the repair response that follows damage to tissues by immune and nonimmune reactions.
摘要
转化生长因子β（TGF-β）在体外对人真皮成纤维细胞是一种有效的趋化因子。完整的二硫键以及可能的TGF-β二聚体结构对其刺激成纤维细胞趋化迁移的能力至关重要，因为用2-巯基乙醇还原会导致其作为趋化因子的效力显著丧失。尽管表皮生长因子（EGF）似乎能够调节TGF-β的某些作用，但它不会改变成纤维细胞对TGF-β的趋化反应。针对同源纯TGF-β的特异性多价兔抗体可阻断其趋化活性，但对其他测试的趋化因子（血小板衍生生长因子、纤连蛋白和变性I型胶原）没有影响。由于TGF-β由多种肿瘤细胞和正常细胞分泌，包括血小板、单核细胞/巨噬细胞和淋巴细胞，它可能在胚胎发生、宿主对肿瘤的反应以及免疫和非免疫反应对组织造成损伤后的修复反应中发挥关键作用。
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