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巴西爬行动物中(血孢子虫目:疟原虫科)寄生虫的分子筛查

Molecular Screening of (Haemosporidia: Plasmodiidae) Parasites from Reptiles in Brazil.

作者信息

Harris D James, Santos Joana L, Borges-Nojosa Diva Maria, de Castro Déborah Praciano

机构信息

CIBIO/InBIO, Universidade do Porto, Centro de Investigação em Biodiversidade e Recursos Genéticos, Campus Agrário de Vairão, 4485-661 Vairão, Portugal.

Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, 4169-007 Porto, Portugal.

出版信息

J Parasitol. 2019 Dec;105(6):913-917.

Abstract

Hemosporidians are a monophyletic group of protozoan parasites infecting all terrestrial vertebrate orders. Although is the most studied genus within the Haemosporidia, this research effort is heavily biased toward mammal and bird hosts. We screened 205 specimens of at least 18 reptile species from Brazil using a partial mitochondrial cytochrome b gene marker. Positive samples were sequenced and included in a phylogenetic assessment. Four positive PCR products matched others identified as using BLAST from 3 different host species, , , and . Recovery of similar haplotypes in the native and exotic (99.9%) indicate potential host-switching.

摘要

血孢子虫是一类感染所有陆生脊椎动物目动物的原生动物寄生虫单系群。虽然疟原虫属是血孢子虫中研究最多的属,但这项研究工作严重偏向于哺乳动物和鸟类宿主。我们使用部分线粒体细胞色素b基因标记物对来自巴西的至少18种爬行动物的205个标本进行了筛查。对阳性样本进行测序并纳入系统发育评估。4个阳性PCR产物与通过BLAST从3种不同宿主物种(鬃狮蜥、巴西珊瑚蛇和巴西彩龟)中鉴定出的其他产物相匹配。在原生鬃狮蜥和外来巴西珊瑚蛇中恢复的相似单倍型(99.9%)表明存在潜在的宿主转换。

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