Laboratório de Neurobiologia da Memória, Departamento de Biofísica, Instituto de Biociências, 91.501-970, Brazil; Laboratório de Estudos sobre Cognição, Memória e Consciência, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Brazil; Programa de Pós-graduação em Neurociências, Instituto de Ciências Básicas da Saúde, 90.046-900, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Laboratório de Neurobiologia da Memória, Departamento de Biofísica, Instituto de Biociências, 91.501-970, Brazil; Programa de Pós-graduação em Neurociências, Instituto de Ciências Básicas da Saúde, 90.046-900, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Neurobiol Learn Mem. 2020 Jan;167:107135. doi: 10.1016/j.nlm.2019.107135. Epub 2019 Dec 9.
Ubiquitination is involved in synaptic plasticity and memory, but the involvement of HECT E3 ligases in these processes has not yet been established. Here, we bilaterally infused heclin, a specific inhibitor of some of these ligases, into the dorsal hippocampus of male Wistar rats that were trained in a contextual fear conditioning. Heclin improved short-term memory, consolidation, retrieval, and reconsolidation when administered immediately post training, prior to testing, or after memory reactivation, respectively. In addition, it impaired memory extinction when administered prior to a long reactivation session. Heclin infusion was also tested for locomotor activity and anxiety-like behavior in a circular arena, but no effect was seen. Taken together, these results indicate that HECT E3 ligases are involved in the modulation of fear memory.
泛素化参与突触可塑性和记忆，但 HECT E3 连接酶在这些过程中的参与尚未确定。在这里，我们将 heclin（一种特定的这些连接酶抑制剂）双侧注入到在情景性恐惧条件反射中接受训练的雄性 Wistar 大鼠的背海马区。当在训练后立即、测试前或记忆重新激活后分别给药时，heclin 分别改善了短期记忆、巩固、检索和再巩固。此外，当在长时间重新激活会话之前给药时，它会损害记忆消退。还在圆形竞技场中测试了 heclin 输注对运动活动和焦虑样行为的影响，但没有观察到效果。总之，这些结果表明 HECT E3 连接酶参与了恐惧记忆的调节。
