College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Guangzhou Quality Supervision and Testing Institute, Guangzhou 511447, China; Research Center of Risk Dynamic Detection and Early Warning for Food Safety of Guangzhou City, Guangzhou 511447, China; Key Laboratory of Detection Technology for Food Safety of Guangzhou City, Guangzhou 511447, China.
Food Chem. 2020 Apr 25;310:125937. doi: 10.1016/j.foodchem.2019.125937. Epub 2019 Dec 3.
Ultrahigh-performance liquid chromatography coupled to quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-HRMS) was used for the screening and determination of 14 heterocyclic aromatic amines (HAAs) in Chinese traditional bacon and sausage. HAAs were extracted from samples using sodium hydroxide solution with acetonitrile, and purified by solid-phase extraction. UHPLC-Q-Orbitrap-HRMS acquired full MS data for quantification, and UHPLC-Q-Orbitrap-Full MS/dd-MS2 (i.e., data-dependent scan mode) obtained product ion spectra for identification. Quantification was achieved using matrix-matched standard calibration curves along with the use of isotope labeled standards as internal standards. Linearity was observed in the range of 0.2-500 μg/L for 14 HAAs, with determination coefficients (R) greater than 0.997. Limits of detection and limits of quantification were in the ranges of 0.1-0.8 μg/kg and 0.3-2.5 μg/kg, respectively. UHPLC-Q-Orbitrap-HRMS demonstrated acceptable performance for quantification and confirmation of HAAs, while UHPLC-Q-Orbitrap-Full MS/dd-MS2 along with library matching showed great potential for screening and confirmation of unknown HAAs in meat products.
超高效液相色谱-四极杆轨道阱高分辨质谱联用(UHPLC-Q-Orbitrap-HRMS)用于筛选和测定中式培根和香肠中的 14 种杂环芳香胺(HAAs)。HAAs 用氢氧化钠溶液和乙腈从样品中提取,并用固相萃取法纯化。UHPLC-Q-Orbitrap-HRMS 采集全 MS 数据进行定量,UHPLC-Q-Orbitrap-Full MS/dd-MS2(即数据依赖扫描模式)获取产物离子谱进行鉴定。采用基质匹配标准校准曲线进行定量,并使用同位素标记标准作为内标。14 种 HAAs 的线性范围为 0.2-500μg/L,相关系数(R)大于 0.997。检出限和定量限分别为 0.1-0.8μg/kg 和 0.3-2.5μg/kg。UHPLC-Q-Orbitrap-HRMS 对 HAAs 的定量和确证具有良好的性能,而 UHPLC-Q-Orbitrap-Full MS/dd-MS2 结合库匹配对肉类产品中未知 HAAs 的筛选和确证具有很大的潜力。