College of Light Industry and Food Sciences, Guangdong Key Laboratory of Science and Technology of Lingnan Special Food, Innovation Research Institute of Modern Agricultural Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Guangzhou Highgoal Biotech Company Limited, Guangzhou 510110, China.
Food Chem. 2021 Nov 1;361:130147. doi: 10.1016/j.foodchem.2021.130147. Epub 2021 May 18.
Triple quadrupole mass spectrometry has been the main technique for HAAs analysis in recent decade, while it requires extensive optimization of compound-dependent parameters. A novel method based on HPLC-Q-Orbitrap-HRMS was developed firstly for simultaneous determination of eighteen HAAs. Extraction and purification conditions were optimized and the developed method was validated in terms of linearity, accuracy and precision. Results indicated eighteen HAAs and two internal standards could be separated in 12 min using a gradient elution program. The full MS/dd-MS scan was adopted for analysis, which indicated favorable recoveries (71.3-114.8%) along with LODs and LOQs in the ranges of 0.02-0.6 and 0.05-2.0 μg/kg, respectively. Internal standards used for calibration could effectively reduce quantification errors produced by matrix effects. The validated method was successfully applied for HAAs analysis in roasted and pan-fried meat and was confirmed to be an alternative method when triple quadrupole mass spectrometry is absent in lab.
三重四极杆质谱法已成为近十年来分析 HAA 的主要技术，但需要对化合物依赖性参数进行广泛优化。本研究首次建立了基于 HPLC-Q-Orbitrap-HRMS 的同时测定十八种 HAA 的新方法。优化了提取和净化条件，并对该方法的线性、准确性和精密度进行了验证。结果表明，采用梯度洗脱程序可在 12 min 内分离出十八种 HAA 和两种内标。采用全 MS/dd-MS 扫描进行分析，表明回收率良好（71.3%-114.8%），检出限和定量限分别在 0.02-0.6 和 0.05-2.0μg/kg 范围内。用于校准的内标可有效降低基质效应引起的定量误差。该方法已成功应用于烤肉和煎肉中的 HAA 分析，当实验室中缺少三重四极杆质谱仪时，该方法可作为替代方法。
