发育中的鸡胚血清蛋白的表达。

Expression of serum proteins in the developing chick embryo.

Sanders B G, Kline K

University of Texas, Department of Zoology, Austin 78712.

Comp Biochem Physiol B. 1977;58(1):97-101. doi: 10.1016/0305-0491(77)90133-x.

Abstract

Sera from different embryonic stages of development and adult chickens were examined for embryonic-adult differences. 2. An antisera prepared in rabbits to 14 day embryonic chick sera revealed an antigen present in the early stages of embryonic development. The antigen reached a peak on day 14 and was not detectable 5 days post hatching. 3. Gel filtration studies in conjunction with immunodiffusion studies revealed the serum antigen to be in the 160,000 mol wt range. 4. Immunoelectrophoretic analyses of embryonic and adult sera revealed the sera components to increase in quantity and in numbers with development. The serum immunoelectrophoretic profile of newly hatch chick serum closely resembled sera from adult chickens. 5. Polyacrylamide gel electrophoretic comparisons of the sera of the developing chicks and the adult chickens revealed proteins specific to certain developmental stages as well as proteins specific to the adult serum. Qualitative and quantitative serum protein differences were found.

摘要

检测了来自不同胚胎发育阶段和成年鸡的血清，以寻找胚胎与成年阶段的差异。2. 用兔制备的针对14日龄胚胎鸡血清的抗血清显示，在胚胎发育早期存在一种抗原。该抗原在第14天达到峰值，孵化后5天无法检测到。3. 凝胶过滤研究结合免疫扩散研究表明，血清抗原的分子量在160,000道尔顿范围内。4. 对胚胎和成年血清的免疫电泳分析显示，血清成分随着发育在数量和种类上增加。新孵化雏鸡血清的免疫电泳图谱与成年鸡血清非常相似。5. 对发育中的雏鸡和成年鸡血清进行聚丙烯酰胺凝胶电泳比较，发现了特定发育阶段特有的蛋白质以及成年血清特有的蛋白质。发现了血清蛋白在定性和定量上的差异。