冬季比目鱼（美洲拟庸鲽）肠道黏膜可溶性蛋白与锌的结合

The binding of zinc to the soluble proteins of intestinal mucosa in winter flounder (Pseudopleuronectes americanus).

Shears M A, Fletcher G L

Marine Sciences Research Laboratory, Memorial University of Newfoundland, St John's, Canada.

Comp Biochem Physiol B. 1979;64(3):297-9. doi: 10.1016/0305-0491(79)90147-0.

Abstract

The binding of Zn2+ to soluble proteins of intestinal mucosa of winter flounder was examined using an equilibrium dialysis technique. 2. There appeared to be more than one binding system present for Zn2+ in the mucosal cytosol. 3. It required four times the normal endogenous Zn2+ level found in the mucosal cytosol to saturate the highest affinity (K1 = 2.42 x 10(7] binding system. 4. Of 10 metals tested Cu2+ was the only one which interfered with Zn2+ binding to the mucosal cytosol proteins. 5. It is postulated that binding proteins in the mucosal cytosol of winter flounder may play a role in the transport of Zn2+.

摘要

使用平衡透析技术检测了锌离子（Zn2+）与冬比目鱼肠黏膜可溶性蛋白质的结合情况。2. 黏膜细胞溶质中似乎存在不止一种锌离子结合系统。3. 需要黏膜细胞溶质中正常内源性锌离子水平的四倍才能使最高亲和力（K1 = 2.42 x 10⁷）的结合系统饱和。4. 在测试的10种金属中，铜离子（Cu2+）是唯一干扰锌离子与黏膜细胞溶质蛋白质结合的金属。5. 据推测，冬比目鱼黏膜细胞溶质中的结合蛋白可能在锌离子的转运中起作用。