冬季比目鱼（美洲拟庸鲽，Walbaum）垂体中促黄体生成激素释放激素（LH-RH）受体的研究。

A study of LH-RH receptors in the pituitary gland of the winter flounder (Pseudopleuronectes americanus Walbaum).

作者信息

Crim L W, St Arnaud R, Lavoie M, Labrie F

机构信息

Marine Sciences Research Laboratory, Memorial University of Newfoundland, St. John's, Canada.

出版信息

Gen Comp Endocrinol. 1988 Mar;69(3):372-7. doi: 10.1016/0016-6480(88)90027-5.

DOI:10.1016/0016-6480(88)90027-5

PMID:2834259

Abstract

A study using an iodinated [D-Ser(tBu)6,Pro9-NHEt]LH-RH (Buserelin), demonstrated the presence of a single class of high-affinity (KD = 2.90 nM), high-capacity LH-RH binding sites in pituitaries obtained from sexually mature male and female winter flounder. Displacement curves for unlabeled Buserelin and other preparations of mammalian and fish LH-RH, but a lack of competition for structurally unrelated peptide hormones, indicated the hormone specific nature of the fish pituitary LH-RH receptor preparation. Compared with native mammalian LH-RH and salmon LH-RH, Buserelin and an analog of salmon LH-RH, [D-Arg6,Trp7,Leu8,Pro9-NHEt]LH-RH, had significantly higher binding affinities for the flounder pituitary receptor correlating with results of previous studies demonstrating the superagonist biological activity of LH-RH analogs in trout and goldfish.

摘要

一项使用碘化的[D-丝氨酸（叔丁基）6，脯氨酸9-乙基]促黄体激素释放激素（布舍瑞林）的研究表明，在从性成熟的雄性和雌性冬比目鱼获取的垂体中存在一类单一的高亲和力（解离常数KD = 2.90 nM）、高容量的促黄体激素释放激素结合位点。未标记的布舍瑞林以及其他哺乳动物和鱼类促黄体激素释放激素制剂的置换曲线，但与结构不相关的肽激素缺乏竞争，表明了鱼类垂体促黄体激素释放激素受体制剂的激素特异性。与天然哺乳动物促黄体激素释放激素和鲑鱼促黄体激素释放激素相比，布舍瑞林和鲑鱼促黄体激素释放激素的类似物[D-精氨酸6，色氨酸7，亮氨酸8，脯氨酸9-乙基]促黄体激素释放激素对鲽鱼垂体受体具有显著更高的结合亲和力，这与先前证明促黄体激素释放激素类似物在鳟鱼和金鱼中具有超级激动剂生物活性的研究结果相关。