比较分析牛母体黄体中小分子 RNA 在晚期妊娠时异常和正常发育的克隆胎儿中的表达。
Comparative analysis of bovine maternal corpus luteum microRNAs with aberrant and normal developed cloned fetus at late gestation.
机构信息
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, 010070, People's Republic of China.
Key Laboratory of Gene Engineering of the Ministry of Education, Guangzhou Key Laboratory of Healthy Aging Research and State Key Laboratory of Biocontrol, SYSU-BCM Joint Research Center, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.
出版信息
Genes Genomics. 2020 Mar;42(3):283-290. doi: 10.1007/s13258-019-00874-y. Epub 2019 Dec 12.
BACKGROUND
The development efficiency of cloned cattle is extremely low (< 5%), most of them were aborted at late gestation. Based on our previous studies, some recipient cows with a cloned fetus would present as engorged uterine vessels and enlarged umbilical vessels randomly. Abortion involves both maternal and fetal factors.
OBJECTIVE
Our aim was to explore this phenomenon by microRNAs expression profile analysis of maternal corpus luteum (CL), which was related to pregnancy maintenance.
METHODS
The present study provided the comparison of maternal CL miRNAs expression of abnormally and normally developed cloned bovine fetus at late gestation (~ 210 days) using RNA-Seq technology.
RESULTS
We selected two abnormally pregnant cows (abnormal group, AG) and three normally pregnant cows (normal group, NG) and acquired valid reads of 9317,261-12,327,185 (~ 84.53-91.28%) from five libraries. In total, we identified 981 conserved miRNAs and 223 novel miRNAs. 1052 miRNAs were co-expressed, 124 miRNAs were uniquely expressed in AG, and 93 miRNAs were uniquely expressed in the NG. Compared with NG, 11 were significantly overexpressed, and 22 were downregulated (p < 0.05) at AG among 1052 co-expressed miRNAs. The differentially expressed miRNAs-targeted genes were further analyzed by Gene Ontology and KEGG pathway analysis. Notably, the steroid biosynthesis pathway was a significantly enriched term (p < 0.01), which may affect the secretion of progesterone.
CONCLUSION
Our research suggested that abnormal miRNAs expression of bovine maternal CL may affect the pregnant status at late gestation.
背景
克隆牛的发育效率极低（<5%），大多数在晚期妊娠时流产。基于我们之前的研究，一些怀有克隆胎儿的受体牛会随机出现子宫血管充盈和脐带血管增大。流产涉及母体和胎儿因素。
目的
我们旨在通过与妊娠维持相关的母体黄体（CL）miRNA 表达谱分析来探讨这一现象。
方法
本研究采用 RNA-Seq 技术比较了晚期妊娠（~210 天）时异常和正常发育的克隆牛胎儿的母体 CL miRNAs 表达。
结果
我们选择了两头异常妊娠的牛（异常组，AG）和三头正常妊娠的牛（正常组，NG），从五个文库中获得了 9317261-12327185（~84.53-91.28%）个有效读数。总共鉴定出 981 个保守 miRNA 和 223 个新 miRNA。1052 个 miRNA 共表达，124 个 miRNA 在 AG 中特异性表达，93 个 miRNA 在 NG 中特异性表达。与 NG 相比，1052 个共表达 miRNA 中有 11 个在 AG 中显著过表达，22 个下调（p<0.05）。进一步对差异表达 miRNA 的靶基因进行 Gene Ontology 和 KEGG 通路分析。值得注意的是，类固醇生物合成途径是一个显著富集的术语（p<0.01），这可能影响孕酮的分泌。
结论
我们的研究表明，牛母体 CL 的异常 miRNA 表达可能会影响晚期妊娠的妊娠状态。
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