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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Department of Biochemistry, University of Auckland, New Zealand.

Comp Biochem Physiol B. 1978;61(1):1-12. doi: 10.1016/0305-0491(78)90204-3.

The beta-N-acetylglucosaminidase (EC 3.2.1.30) activities of human, pig, calf, lamb, rat and rabbit liver and plasma have been investigated. 2. All preparations had maximum activity between pH 4.0 and 4.5 and Km values with the substrate 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside ranged from 0.54 to 2.54 mM. 3. The isoenzyme profiles of liver and plasma beta-N-acetylglucosaminidase activity were compared using DEAE-cellulose chromatography. In all species the major anionic component of liver (beta-N-acetylglucsaminidase A) was eluted at a higher salt concentration than the most anionic plasma isoenzyme. 4. The plasma beta-N-acetylglucosaminidase A isoenzyme of all species contained sialic acid residues whereas only the rabbit, pig and calf liver isoenzymes were sialylated.

对人、猪、小牛、羔羊、大鼠和兔子的肝脏及血浆中的β-N-乙酰氨基葡萄糖苷酶（EC 3.2.1.30）活性进行了研究。2. 所有制剂在pH 4.0至4.5之间具有最大活性，以4-甲基伞形酮基-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖苷为底物时的米氏常数（Km值）范围为0.54至2.54 mM。3. 使用DEAE-纤维素色谱法比较了肝脏和血浆中β-N-乙酰氨基葡萄糖苷酶活性的同工酶谱。在所有物种中，肝脏的主要阴离子成分（β-N-乙酰氨基葡萄糖苷酶A）在比最阴离子性的血浆同工酶更高的盐浓度下被洗脱。4. 所有物种的血浆β-N-乙酰氨基葡萄糖苷酶A同工酶都含有唾液酸残基，而只有兔子、猪和小牛的肝脏同工酶被唾液酸化。

Department of Biochemistry, University of Auckland, New Zealand.

Comp Biochem Physiol B. 1978;61(1):1-12. doi: 10.1016/0305-0491(78)90204-3.

The beta-N-acetylglucosaminidase (EC 3.2.1.30) activities of human, pig, calf, lamb, rat and rabbit liver and plasma have been investigated. 2. All preparations had maximum activity between pH 4.0 and 4.5 and Km values with the substrate 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside ranged from 0.54 to 2.54 mM. 3. The isoenzyme profiles of liver and plasma beta-N-acetylglucosaminidase activity were compared using DEAE-cellulose chromatography. In all species the major anionic component of liver (beta-N-acetylglucsaminidase A) was eluted at a higher salt concentration than the most anionic plasma isoenzyme. 4. The plasma beta-N-acetylglucosaminidase A isoenzyme of all species contained sialic acid residues whereas only the rabbit, pig and calf liver isoenzymes were sialylated.

对人、猪、小牛、羔羊、大鼠和兔子的肝脏及血浆中的β-N-乙酰氨基葡萄糖苷酶（EC 3.2.1.30）活性进行了研究。2. 所有制剂在pH 4.0至4.5之间具有最大活性，以4-甲基伞形酮基-2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖苷为底物时的米氏常数（Km值）范围为0.54至2.54 mM。3. 使用DEAE-纤维素色谱法比较了肝脏和血浆中β-N-乙酰氨基葡萄糖苷酶活性的同工酶谱。在所有物种中，肝脏的主要阴离子成分（β-N-乙酰氨基葡萄糖苷酶A）在比最阴离子性的血浆同工酶更高的盐浓度下被洗脱。4. 所有物种的血浆β-N-乙酰氨基葡萄糖苷酶A同工酶都含有唾液酸残基，而只有兔子、猪和小牛的肝脏同工酶被唾液酸化。