Kim Young Il, Lee Chan-Yang, Shin Min Kyung
Medical Science Research Institute, Kyung Hee University Medical Center, Seoul 02447, Republic of Korea.
Department of Dermatology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Biomed Rep. 2020 Jan;12(1):17-22. doi: 10.3892/br.2019.1258. Epub 2019 Nov 21.
Activins are members of the transforming growth factor-β (TGF-β) superfamily and play important roles in proliferation, differentiation, and apoptosis of various target cells. We investigated changes of activin, activin receptor (ActR), and Smad-signaling gene expression with increasing passage number in normal human dermal fibroblasts. The expression of mRNA and protein was measured by reverse transcription-quantitative polymerase chain reaction and immunoblot analysis from passage numbers 5 to 15. Activin A and follistatin transcript levels increased with increasing passage number. ActR types IA, IB, IIA and IIB mRNA levels decreased at high passage number. The levels of Smad2, 3 and 4 protein decreased with increasing passage number, which also attenuated phosphorylation of Smad2 and 3 protein expression. Smad7 was enhanced with increasing passage number. These results suggest that expression of activin-signaling in aging normal human dermal fibroblasts increases activin A and follistatin, whereas ActR-Smad signaling is decreased.
激活素是转化生长因子-β（TGF-β）超家族的成员，在各种靶细胞的增殖、分化和凋亡中发挥重要作用。我们研究了正常人皮肤成纤维细胞中激活素、激活素受体（ActR）和Smad信号基因表达随传代次数增加的变化。通过逆转录定量聚合酶链反应和免疫印迹分析，测定了第5至15代细胞中mRNA和蛋白质的表达。激活素A和卵泡抑素转录水平随传代次数增加而升高。高传代时，ActR IA、IB、IIA和IIB型mRNA水平降低。Smad2、3和4蛋白水平随传代次数增加而降低，这也减弱了Smad2和3蛋白表达的磷酸化。Smad7随传代次数增加而增强。这些结果表明，衰老的正常人皮肤成纤维细胞中激活素信号的表达增加了激活素A和卵泡抑素，而ActR-Smad信号则降低。
